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EOMES Antibody (BLR104H) - BSA Free

Novus Biologicals | Catalog # NBP3-07014

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR104H

Format

BSA Free

View all EOMES Primary Antibodies »

Product Specifications

Immunogen

Immunogen was a peptide representing a region between residue 125 and 175 of human eomesodermin homolog (EOMES) using the numbering given in entry NP_001265111.1 (Gene ID 8320).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EOMES Antibody (BLR104H) - BSA Free

Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]

Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Whole cell lysate (10 ug) from HEK293T, HeLa, NK-92, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

Immunocytochemistry/ Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014]

Immunocytochemistry/Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in NK-92 cells by ICC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014]

Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in tonsil by IHC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRP-conjugatedgoat anti-rabbit IgG. Substrate: DAB.

Western Blot: EOMES Antibody (BLR104H) [NBP3-07014]

Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Detection of human EOMES by WB of immunoprecipitates from NK-92 cell lysate. Antibodies: NBP3-07014 and rabbit anti-EOMES BL24367.

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Applications for EOMES Antibody (BLR104H) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

6 ul/1 mg lysate

Western Blot

1:1000

Application Notes

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Borate Buffered Saline, pH 8.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: EOMES

EOMES encodes a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation.

Long Name

Eomesodermin Homolog

Alternate Names

Eomesodermin, TBR2

Gene Symbol

EOMES

Additional EOMES Products

Product Documents for EOMES Antibody (BLR104H) - BSA Free

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Certificate of Analysis

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Product Specific Notices for EOMES Antibody (BLR104H) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.