epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Novus Biologicals | Catalog # NBP2-41373
![Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373]](https://resources.rndsystems.com/images/products/epithelial-Sodium-Channel-gamma-Antibody-5c2-BSA-Free-Immunohistochemistry-Paraffin-NBP2-41373-img0003.jpg "Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5c2
Format
BSA Free
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Product Specifications
Immunogen
The inhibitory peptide from the human yENaC subunit. EAESWNSVSEGKQPRFSHRIPLC corresponding to
amino acid residue 139-160 of human yENaC subunit.
Epitope
The epitope is on the inhibitory tract of human yENaC.
Specificity
Antibody is specific for the inhibitory tract of human gENaC.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373]
Immunohistochemistry-Paraffin: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. Staining of Human kidney tissue. Courtesy of Per Svenningsen, PhD.
Sandwich ELISA: epithelial Sodium Channel gamma Antibody (5c2) - BSA Free [NBP2-41373] - Analysis using the Biotin conjugate of NBP2-41373. The calibration curveof a sandwich assay using NBP2-41371 as the capture antibody and NBP2-41372B as the biotinylated detection antibody.
Applications for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: A sandwich ELISA can be made using NBP2-41371 (1-4 ug/ml) as the capture antibody and biotinylated NBP2-41373 (0.05 - 0.2 ug/ml) as the detection antibody in order to detect the peptide from the inhibitory tract (AA 138-131).
WB: NBP2-41373 was used in Western blot. (1)
IHC: NBP2-41373 was used in immunohistochemistry. (1)
IF: NBP2-41373 was used in immunofluorescence. (1)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: epithelial Sodium Channel gamma
Long Name
Amiloride-sensitive sodium channel subunit gamma
Alternate Names
ENaCG, Gamma-ENaC, Gamma-NaCH, SCNEG, SCNN1G
Gene Symbol
SCNN1G
Additional epithelial Sodium Channel gamma Products
Product Documents for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for epithelial Sodium Channel gamma Antibody (5c2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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