EPRS Antibody - BSA Free

Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929]

Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929]

Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.

Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929]

Immunohistochemistry-Paraffin: EPRS Antibody [NBP1-84929] - Staining of human endometrium shows moderate cytoplasmic postivity in glandular cells.

Western Blot: EPRS Antibody - BSA Free [NBP1-84929] -

Identification of MA residues critical for interaction with EPRS. (A) Alanine-scanning mutagenesis screen to identify interaction-ablative MA mutants. MA residues were mutated to alanine in groups of three. A total of 43 mutants were generated and tested for EPRS interaction via co-IP. Five mutants with robust MA expression but significantly reduced EPRS interaction are indicated with red boxes. EPRS IP/input ratio (normalized to WT) is labeled below each lane. Mutations of interest were selected based on two criteria: (1) low IP/input ratio; (2) MA-FLAG expression is comparable to WT. (B) Production of progeny virions by MA mutants as measured via luciferase activity in producer cells (top). Gag expression in producer cells and HIV-1 CA in supernatants as assessed by immunoblotting (bottom). The top of the bars represents mean value of 3 trials. (C) Relative infectivity of virion-containing supernatants measured by luciferase activity in target cells. The top of the bars represents mean value of 3 trials. (D) Critical interacting residues (orange) mapped onto a previously reported crystal structure of MA binding to PI(4,5)P2 (PDB: 2H3Z) [52]. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36851687), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: EPRS Antibody - BSA Free [NBP1-84929] -

EPRS protein levels are not altered in cells infected with single-cycle HIV-1. (A) Immunoblot showing level of EPRS in HEK293T cells uninfected or infected with HIV-Luc/VSV-G with M.O.I. of 5. (B) Immunoblot showing level of EPRS in HuT/CCR5 cells uninfected or infected with HIV-Luc/VSV-G with MOI of 0.5 (+) or 2.5 (++). Graph shows level of infectivity as measured by luciferase production. (C) Immunofluorescence imaging of Gag-GFP and EPRS expression in HeLa cells infected with HIV-Luc/VSV-G. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36851687), licensed under a CC-BY license. Not internally tested by Novus Biologicals.