ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) - BSA Free

Immunohistochemistry: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] -

Immunohistochemistry: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Image of ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] -

Western Blot: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Positive Western Blot detected in A549 whole cell lysate, HepG2 whole cell lysate (treated with EGF or not).
All lanes: ERK1/ERK2 [p Thr202, p Thr185] Antibody at 2.35ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 42 KDa
Observed band size: 42 KDa

Immunocytochemistry/Immunofluorescence: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] -

Immunocytochemistry/Immunofluorescence: ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) [NBP3-26412] - Staining of Hela cells with ERK1/ERK2 [p Thr202, p Thr185] Antibody (2D5) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).