ERp57/PDIA3 Antibody (CL2444) - BSA Free

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U2OS cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) - BSA Free [NBP2-36765] -

The expression of VDR and PDIA3/1,25MARRS in cortical neurons.A-B) Cell surface staining of VDR (green) in live neurons, followed by fixation and immunofluorescence labeling of MAP2 (red) as a neuronal marker, 100x. The micrographs were taken from the same areas with different levels of focus to demonstrate the different localization of VDR on the neuronal plasma membrane. A) VDR protein is localized on the plasma membrane of the soma; B) VDR protein is localized on the plasma membranes of neurites. The 3D image of staining was obtained via confocal microscopy (63x), and the video is presented in S1 Video. C) VDR (green) and PDIA3/1,25MARRS (red) cell surface staining with live neurons via double immunofluorescence labelling, 100x. The localization of PDIA3/1,25MARRS on the neuronal plasma membrane was very limited. D) Double immunofluorescence labelling of VDR and PDIA3/1,25MARRS in fixed and permeabilized neurons, 100x. PDIA3/1,25MARRS was localized in the cytoplasm and in the endoplasmic reticulum (ER). Given its strong reactivity in a certain area in the cytoplasm and its known role as an ER chaperone, the localization was considered to be in the ER. VDR is located in the nucleus, cytoplasm, ER and axon hillock. VDR and PDIA3/1,25MARRS might co-localize in the cytoplasm, especially in the ER. E) VDR (green) cell surface staining with live SH-SY5Y cells via immunofluorescence labelling, 100x. F) Immunofluorescence labelling of VDR in fixed and permeabilized SH-SY5Y cells, 100x. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29176823), licensed under a CC-BY license. Not internally tested by Novus Biologicals.