ERp57/PDIA3 Antibody (CL2444) - BSA Free

Novus Biologicals | Catalog # NBP2-36765

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2444

Format

BSA Free
View all ERp57/PDIA3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from G5EA52, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse ERp57/PDIA3 Antibody (CL2444) - BSA Free (NBP2-36765) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ERp57/PDIA3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ERp57/PDIA3 Antibody (CL2444) - BSA Free

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in human thyroid gland and skeletal muscle tissues. Corresponding PDIA3 RNA-seq data are presented for the same tissues.
Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PDIA3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Western Blot: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Lane 1: Marker [kDa]. Lane 2:Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/ Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining in U2OS cell line with Anti-PDIA3 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human thyroid gland shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765]

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody (CL2444) [NBP2-36765] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Applications for ERp57/PDIA3 Antibody (CL2444) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20000 - 1:50000

Immunohistochemistry-Paraffin

1:20000 - 1:50000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ERp57/PDIA3

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Long Name

Endoplasmic Reticulum Resident Protein 57

Alternate Names

ERp60, GRP58, PDIA3

Gene Symbol

PDIA3

UniProt

G5EA52 (Human)

Additional ERp57/PDIA3 Products

Product Documents for ERp57/PDIA3 Antibody (CL2444) - BSA Free

Certificate of Analysis

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Product Specific Notices for ERp57/PDIA3 Antibody (CL2444) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Redox Enzymes

Citations for ERp57/PDIA3 Antibody (CL2444) - BSA Free

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Protocols

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