ETFA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84856

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ETFA Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ETFA Antibody - BSA Free

Western Blot: ETFA Antibody [NBP1-84856]

Western Blot: ETFA Antibody [NBP1-84856]

Western Blot: ETFA Antibody [NBP1-84856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: ETFA Antibody [NBP1-84856]

Immunocytochemistry/ Immunofluorescence: ETFA Antibody [NBP1-84856]

Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP1-84856] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856] - Staining of human liver.
Western Blot: ETFA Antibody [NBP1-84856]

Western Blot: ETFA Antibody [NBP1-84856]

Western Blot: ETFA Antibody [NBP1-84856] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856] - Staining of human colon shows strong cytoplasmic positivity with a granular pattern, in glandular cells.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856] - Staining of human colon, kidney, liver and pancreas using Anti-ETFA antibody NBP1-84856 (A) shows similar protein distribution across tissues to independent antibody NBP1-84855 (B).
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856] - Staining of human pancreas.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856] - Staining of human kidney.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84856] - Staining of human colon.

Applications for ETFA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ETFA

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alpha-ETF, electron transfer flavoprotein alpha-subunit, Electron transfer flavoprotein subunit alpha, mitochondrial, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, Glutaric Aciduria II, MADD

Gene Symbol

ETFA

Additional ETFA Products

Product Documents for ETFA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ETFA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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