ETFA Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16382

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ETFA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ETFA Antibody - BSA Free

Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ETFA Antibody [NBP2-16382]

Immunocytochemistry/ Immunofluorescence: ETFA Antibody [NBP2-16382]

Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.
Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382] - 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000.
Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382]

Western Blot: ETFA Antibody [NBP2-16382] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody dilution: 1:500.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382]

Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody dilution: 1:500.
Immunoprecipitation: ETFA Antibody [NBP2-16382]

Immunoprecipitation: ETFA Antibody [NBP2-16382]

Immunoprecipitation: ETFA Antibody [NBP2-16382] - Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 ug of ETFA antibody. Western blot analysis was performed using ETFA antibody.

Applications for ETFA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ETFA

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alpha-ETF, electron transfer flavoprotein alpha-subunit, Electron transfer flavoprotein subunit alpha, mitochondrial, electron-transfer-flavoprotein, alpha polypeptide, EMA, GA2, Glutaric Aciduria II, MADD

Gene Symbol

ETFA

UniProt

P13804 (Human)

Additional ETFA Products

Product Documents for ETFA Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ETFA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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