Exosome Component 9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-71702

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human, Mouse, Avian - Chicken

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Exosome Component 9 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal region of human Exosome Component 9 (within residues 250-439). [Swiss-Prot Q06265]

Reactivity Notes

Immunogen has 83% identity to rat and 90% identity to bovine.

Localization

Cytoplasm. Nucleus - nucleolus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Exosome Component 9 Antibody - BSA Free

Western Blot: Exosome Component 9 AntibodyBSA Free [NBP1-71702]

Western Blot: Exosome Component 9 AntibodyBSA Free [NBP1-71702]

Western Blot: Exosome Component 9 Antibody [NBP1-71702] - Analysis of EXOSC9 in A. HepG2 cell lysate and B. MCF7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunocytochemistry/ Immunofluorescence: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunocytochemistry/Immunofluorescence: Exosome Component 9 Antibody [NBP1-71702] - EXOSC9 antibody was tested at 1:100 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunohistochemistry: Exosome Component 9 Antibody - BSA Free [NBP1-71702]

Immunohistochemistry: Exosome Component 9 Antibody [NBP1-71702] - Staining of EXOSC9 in mouse prostate.
Simple Western: Exosome Component 9 AntibodyBSA Free [NBP1-71702]

Simple Western: Exosome Component 9 AntibodyBSA Free [NBP1-71702]

Simple Western: Exosome Component 9 Antibody [NBP1-71702] - Simple Western lane view shows a specific band for Exosome Component 9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Exosome Component 9 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 27543448)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Immunoprecipitation

reported by customer review

Simple Western

1:1000

Western Blot

1:5000
Application Notes

In Western blot, a band is seen ~75 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HepG2 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 62 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-71702 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Exosome Component 9

Exosome component 9 is part of the 3' -> 5' exoribonuclease complex that helps to degrade unstable mRNA. It binds to ARE-containing RNAs and performs 3' end trimming and degradation. These activities lead toward the maturation of RNA into more stable forms, which then become involved in transcription and translation. In the related Arabidopsis equivalent, loss of function of this protein is a lethal mutation. In ruminants, EXOSC9 is differentially expressed throughout the gut at different stages of development. EXOSC9 seems to be important in the degradation of histone mRNA and may be important in some downstream epigenetic effects.

Alternate Names

p5, p6, PCH1D, PM/Scl-75, PMSCL1, RRP45, Rrp45p

Entrez Gene IDs

5393 (Human); 50911 (Mouse)

Gene Symbol

EXOSC9

UniProt

Q06265 (Human), Q9JHI7 (Mouse)

Additional Exosome Component 9 Products

Product Documents for Exosome Component 9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Exosome Component 9 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Exosome Component 9 Antibody - BSA Free

Customer Reviews for Exosome Component 9 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
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  • Name: Hsin Jing-Ping
    Application: Western Blot
    Sample Tested:
    Species: Other
    Verified Customer | Posted 10/18/2013
    Exosome Component 9 Antibody - BSA Free NBP1-71702
  • Name: Patricia Richard
    Application: Immunoprecipitation
    Sample Tested: HeLa, U87 whole cell extract and nuclear extract
    Species: Human
    Verified Customer | Posted 08/02/2012
    Exosome Component 9 Antibody - BSA Free NBP1-71702

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Protocols

View specific protocols for Exosome Component 9 Antibody - BSA Free (NBP1-71702):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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FAQs

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