EZH2/KMT6 Antibody (EZH2/2536)
Novus Biologicals | Catalog # NBP3-07187
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # EZH2/2536
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Product Specifications
Immunogen
Recombinant full-length human EZH2/KMT6 protein (Uniprot: Q15910)
Localization
Nucleus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08932)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for EZH2/KMT6 Antibody (EZH2/2536)
![Western Blot: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187]](https://resources.rndsystems.com/images/products/EZH2-KMT6-Antibody-EZH2-2536-Western-Blot-NBP3-07187-img0002.jpg "Western Blot: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187]")
Western Blot: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187]
Western Blot: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187] - Western Blot Analysis of MCF-7 cell lysate using EZH2/KMT6 Mouse Monoclonal Antibody (EZH2/2536).![Protein Array: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187]](https://resources.rndsystems.com/images/products/EZH2-KMT6-Antibody-EZH2-2536-Protein-Array-NBP3-07187-img0001.jpg "Protein Array: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187]")
Protein Array: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187]
Protein Array: EZH2/KMT6 Antibody (EZH2/2536) [NBP3-07187] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EZH2/KMT6 Mouse Monoclonal Antibody (EZH2/KMT6/2536).
EZH2/KMT6 Antibody (EZH2/2536)
Immunofluorescence Analysis of HeLa cells using EZH2/KMT6 antibody (EZH2/2536) followed by goat anti-mouse IgG-CF488 (green); counterstain (RedDot).
EZH2/KMT6 Antibody (EZH2/2536)
Flow Cytometric Analysis of PFA-fixed HeLa cells. EZH2/KMT6 antibody (EZH2/2536) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).Applications for EZH2/KMT6 Antibody (EZH2/2536)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: EZH2
Long Name
Enhancer of Zeste Homolog 2 [Drosophila]
Alternate Names
ENX1, KMT6
Gene Symbol
EZH2
UniProt
Additional EZH2 Products
Product Documents for EZH2/KMT6 Antibody (EZH2/2536)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for EZH2/KMT6 Antibody (EZH2/2536)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
