FABP2/I-FABP Antibody (9A9B7B3)

Novus Biologicals | Catalog # NBP1-51589

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9A9B7B3
View all FABP2/I-FABP Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human FABP2/I-FABP expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FABP2/I-FABP Antibody (9A9B7B3)

Western Blot: FABP2/I-FABP Antibody (9A9B7B3)BSA Free [NBP1-51589]

Western Blot: FABP2/I-FABP Antibody (9A9B7B3)BSA Free [NBP1-51589]

Western Blot: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis using FABP2 mouse mAb against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: FABP2/I-FABP Antibody (9A9B7B3) - BSA Free [NBP1-51589]

Immunocytochemistry/ Immunofluorescence: FABP2/I-FABP Antibody (9A9B7B3) - BSA Free [NBP1-51589]

Immunocytochemistry/Immunofluorescence: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis of 3T3-L1 cells using FABP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (9A9B7B3) - BSA Free [NBP1-51589]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (9A9B7B3) - BSA Free [NBP1-51589]

Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Analysis of paraffin-embedded human Small Intestine tissues.
Flow Cytometry: FABP2/I-FABP Antibody (9A9B7B3) - BSA Free [NBP1-51589]

Flow Cytometry: FABP2/I-FABP Antibody (9A9B7B3) - BSA Free [NBP1-51589]

Flow Cytometry: FABP2/I-FABP Antibody (9A9B7B3) [NBP1-51589] - Flow cytometric analysis of LOVO cells using FABP2 mouse mAb (green) and negative control (purple).

Applications for FABP2/I-FABP Antibody (9A9B7B3)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FABP2/I-FABP

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq]

Long Name

Fatty Acid-Binding Protein 2/Intestinal FABP

Alternate Names

I-FABP, IFABP, Intestinal FABP

Gene Symbol

FABP2

Additional FABP2/I-FABP Products

Product Documents for FABP2/I-FABP Antibody (9A9B7B3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FABP2/I-FABP Antibody (9A9B7B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Citations for FABP2/I-FABP Antibody (9A9B7B3)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FABP2/I-FABP Antibody (9A9B7B3)

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  • Q: In your product page of FABP2/I-FABP Antibody (9A9B7B3), FABP2 is detected at the molecular size around 17kDa in LOVO cells. Overexpressed FABP2 was detected at the around 43kDa in HEK293 cells as a single band without lower size band. I wonder why over expressed FABP2 is detected at 42-43kDa without 17kDa band. Do you have any explanation for this finding?

    A: The Western blot shown on our datasheet is of an analysis using FABP2 mouse mAb against FABP2-hIgGFc transfected HEK293 (1) cell lysate and LOVO (2) cell lysate.  Fc tag alon is 26 kDa and with the 15 kDa FABP2 sequence, Fc-fused FABP2 runs at ~41 kDa.   The protein alone should run at ~15 kDa.

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