FABP4/A-FABP Antibody (JM10-99)
Novus Biologicals | Catalog # NBP2-66828
Recombinant Monoclonal Antibody
![Western Blot: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]](https://resources.rndsystems.com/images/products/FABP4-A-FABP-Antibody-JM10-99-Western-Blot-NBP2-66828-img0005.jpg "Western Blot: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JM10-99 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within human FABP4/A-FABP aa 60-100. (SwissProt: P15090 Human; SwissProt: P04117 Mouse; SwissProt: P70623 Rat)
Localization
Nucleus. Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FABP4/A-FABP Antibody (JM10-99)
Western Blot: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]
Western Blot: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of FABP4 on mouse heart tissue lysates using anti-FABP4 antibody at 1/500 dilution.![Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]](https://resources.rndsystems.com/images/products/FABP4-A-FABP-Antibody-JM10-99-Immunocytochemistry-Immunofluorescence-NBP2-66828-img0006.jpg "Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]")
Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]
Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in PMVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]](https://resources.rndsystems.com/images/products/FABP4-A-FABP-Antibody-JM10-99-Immunohistochemistry-Paraffin-NBP2-66828-img0004.jpg "Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]")
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of paraffin-embedded mouse lung tissue using anti-FABP4 antibody. Counter stained with hematoxylin.![Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]](https://resources.rndsystems.com/images/products/FABP4-A-FABP-Antibody-JM10-99-Immunocytochemistry-Immunofluorescence-NBP2-66828-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]")
Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]
Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]](https://resources.rndsystems.com/images/products/FABP4-A-FABP-Antibody-JM10-99-Immunocytochemistry-Immunofluorescence-NBP2-66828-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]")
Immunocytochemistry/ Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]
Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]](https://resources.rndsystems.com/images/products/FABP4-A-FABP-Antibody-JM10-99-Immunohistochemistry-Paraffin-NBP2-66828-img0003.jpg "Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]")
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828]
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of paraffin-embedded human breast tissue using anti-FABP4 antibody. Counter stained with hematoxylin.Applications for FABP4/A-FABP Antibody (JM10-99)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:500
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FABP4/A-FABP
Long Name
Fatty Acid-Binding Protein 4
Alternate Names
A-FABP, AFABP, aP2
Gene Symbol
FABP4
Additional FABP4/A-FABP Products
Product Documents for FABP4/A-FABP Antibody (JM10-99)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FABP4/A-FABP Antibody (JM10-99)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
