FABP4/A-FABP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89218

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYE

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31518157).

Marker

Adipocyte Differentiation Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FABP4/A-FABP Antibody - BSA Free

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Analysis in human adipose tissue and cerebral cortex tissues. Corresponding FABP4 RNA-seq data are presented for the same tissues.
Western Blot: FABP4/A-FABP Antibody [NBP1-89218]

Western Blot: FABP4/A-FABP Antibody [NBP1-89218]

Western Blot: FABP4/A-FABP Antibody [NBP1-89218] - Analysis in human adipose tissue tissue.
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human adipose tissue shows strong cytoplasmic positivity in adipocytes.
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human cerebral cortex shows no cytoplasmic positivity in neurons as expected.
Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218]

Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody [NBP1-89218] - Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.
Simple Western: FABP4/A-FABP Antibody [NBP1-89218]

Simple Western: FABP4/A-FABP Antibody [NBP1-89218]

Simple Western: FABP4/A-FABP Antibody [NBP1-89218] - Simple Western lane view shows a specific band for FABP4 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: FABP4/A-FABP Antibody [NBP1-89218]

Simple Western: FABP4/A-FABP Antibody [NBP1-89218]

Simple Western: FABP4/A-FABP Antibody [NBP1-89218] - Electropherogram image(s) of corresponding Simple Western lane view. FABP4/A-FABP antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).
FABP4/A-FABP Antibody - BSA Free

Western Blot: FABP4/A-FABP Antibody - BSA Free [NBP1-89218] -

Correlation between reduced iron levels and increased ferroportin expression in injured muscles. (a) Histological difference between intact control (CTL) muscle and injured muscle by hematoxylin and eosin staining. Pictures are representative of the muscle tissue from pronator quadratus (hand) and rotator cuff (shoulder) muscles (n = 10 per group). Scale bar: 100 μm. (b) Intracellular iron concentration between intact control and injured muscles (n = 30 per group). (c) mRNA expression of ferritin, hepcidin and ferroportin for intact control and injured muscles (n = 30 per group). (d) Western blot analyses for myosin heavy chain (MYH), fatty acid binding protein 4 (FABP4), ferritin, ferroportin and alpha -tubulin protein expression in intact control and injured muscles (n = 6 per group). A densitometric analysis of the Western blots is shown in the graph on the right. (e) Immunofluorescence (IF) microscopy images showing ferroportin expression. The blue signals indicate cell nuclei. Images are representative of independent tissue samples (n = 10 per group). Scale bar: 100 μm. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). Ferroportin expression levels (green staining) were assessed with densitometry using Image J software (Version 1.8.0, National Institutes of Health, Bethesda, MD, USA), shown on the right. Data represent mean +/- SEM. * p < 0.05. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36139428), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FABP4/A-FABP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 18 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FABP4/A-FABP

Fatty acid binding protein 4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism.

Long Name

Fatty Acid-Binding Protein 4

Alternate Names

A-FABP, AFABP, aP2

Gene Symbol

FABP4

Additional FABP4/A-FABP Products

Product Documents for FABP4/A-FABP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FABP4/A-FABP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FABP4/A-FABP Antibody - BSA Free

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Protocols

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