FABP7/B-FABP Antibody (2A84) - BSA Free
Novus Biologicals | Catalog # NBP3-05531
![Western Blot: FABP7/B-FABP Antibody [NBP3-05531]](https://resources.rndsystems.com/images/products/FABP7-B-FABP-Antibody-Western-Blot-NBP3-05531-img0002.jpg "Western Blot: FABP7/B-FABP Antibody [NBP3-05531]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2A84
Format
BSA Free
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Product Specifications
Immunogen
Recombinant full length human FABP7/B-FABP expressed in and purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FABP7/B-FABP Antibody (2A84) - BSA Free
Western Blot: FABP7/B-FABP Antibody [NBP3-05531]
Western Blot: FABP7/B-FABP Antibody [NBP3-05531] - Western blot analysis of whole brain and neuron-glial cell culture lysates using FABP7/B-FABP Antibody at a at a dilution of of 1:1,000 in green, and chicken pAb to HSP60 at a dilution of of 1:10,000 in red: [1] protein standard, [2] adult rat brain, [3] embryonic (E18) rat brain, and [4] rat neuron-glial cells lysates. The bands at ~14kDa correspond to FABP7/B-FABP detected only in developing tissue and cells, and 60kDa bands represent HSP60 protein, which is labeled in all preparations.![Immunocytochemistry/ Immunofluorescence: FABP7/B-FABP Antibody [NBP3-05531]](https://resources.rndsystems.com/images/products/FABP7-B-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05531-img0001.jpg "Immunocytochemistry/ Immunofluorescence: FABP7/B-FABP Antibody [NBP3-05531]")
Immunocytochemistry/ Immunofluorescence: FABP7/B-FABP Antibody [NBP3-05531]
Immunocytochemistry/Immunofluorescence: FABP7/B-FABP Antibody [NBP3-05531] - Immunofluorescent analysis rat embryonic brain section stained with mouse FABP7/B-FABP at a at a dilution of of 1:500 in green, and costained with chicken pAb to vimentin at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. FABP7/B-FABP antibody stains developing astrocytes and radial glia, while vimentin antibody reveals cytoplasmic intermediate filaments. Since the sub cellular distribution of these two proteins is distinct they expression patterns overlap but are not identical.
Immunohistochemistry-Frozen: Mouse FABP7/B-FABP mAb (2A84) [NBP3-05531]
FABP7 is increased in progressive multiple sclerosis and induces a pro-inflammatory phenotype in monocytes through a glycolytic switch. Image from a verified customer review. The customer used the AF488-conjugated version of NBP3-05531, which is listed under NBP3-05531AF488.Applications for FABP7/B-FABP Antibody (2A84) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry
1:1000
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: FABP7/B-FABP
Long Name
Fatty Acid-Binding Protein 7
Alternate Names
B-FABP, BFABP, BLBP
Gene Symbol
FABP7
Additional FABP7/B-FABP Products
Product Documents for FABP7/B-FABP Antibody (2A84) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FABP7/B-FABP Antibody (2A84) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for FABP7/B-FABP Antibody (2A84) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
