Family with Sequence Similarity 105, Member B (FAM105B), also known as OTULIN, is a 352 amino acid (aa) member of the ovarian tumor protein superfamily with a predicted molecular weight of 40 kDa. The mouse FAM105B/OTULIN ortholog shares 89% aa sequence identity with the human protein. It is a C65 type peptidase that has high specificity for methionine-1-linked linear Ubiquitin chains and contributes to the regulation of NFkB signaling.
FAM105B/OTULIN Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-14722
![Western Blot: FAM105B/OTULIN Antibody [NBP2-14722]](https://resources.rndsystems.com/images/products/FAM105B-OTULIN-Antibody-Western-Blot-NBP2-14722-img0009.jpg "Western Blot: FAM105B/OTULIN Antibody [NBP2-14722]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (93%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: DEIEKEKELLIHERGASEPRLSVAPEMDIMDYCKKEWRGNTQKATCMKMGYEEVSQKFTSIRRVRGDNYCALRATLFQAMSQAVGLPPWLQDPELMLLP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAM105B/OTULIN Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722]](https://resources.rndsystems.com/images/products/FAM105B-OTULIN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14722-img0005.jpg "Immunocytochemistry/ Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722]")
Immunocytochemistry/ Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722]
Immunocytochemistry/Immunofluorescence: FAM105B/OTULIN Antibody [NBP2-14722] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & mitochondria.![Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722]](https://resources.rndsystems.com/images/products/FAM105B-OTULIN-Antibody-Immunohistochemistry-Paraffin-NBP2-14722-img0001.jpg "Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722]")
Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722]
Immunohistochemistry-Paraffin: FAM105B/OTULIN Antibody [NBP2-14722] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
FAM105B/OTULIN Antibody [NBP2-14722] -
Staining of human liver shows strong cytoplasmic granular positivity in hepatocytes.
FAM105B/OTULIN Antibody [NBP2-14722] -
Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.
FAM105B/OTULIN Antibody [NBP2-14722]
Staining of human kidney shows strong cytoplasmic granular positivity in cells in tubules.Applications for FAM105B/OTULIN Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FAM105B/OTULIN
Long Name
Family with Sequence Similarity 105, Member B
Alternate Names
OTULIN
Gene Symbol
OTULIN
Additional FAM105B/OTULIN Products
Product Documents for FAM105B/OTULIN Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FAM105B/OTULIN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for FAM105B/OTULIN Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
