FARSLA Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88067

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FARSLA Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVIEAELRSTKHWELTAEGEEIAREGSHEARVFRSIPPEGLAQSELMRLPSGKVGFSKAMSNKWIRVDKSAADGPRVFRVVDSMEDEVQRRLQLVRGGQAEKLGEKERSELRKRKLLAEVTLKTYWVSKG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FARSLA Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FARSLA Antibody [NBP1-88067]

Immunocytochemistry/ Immunofluorescence: FARSLA Antibody [NBP1-88067]

Immunocytochemistry/Immunofluorescence: FARSLA Antibody [NBP1-88067] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Analysis in human cerebral cortex and liver tissues. Corresponding FARSA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human cerebral cortex shows very strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067]

Immunohistochemistry-Paraffin: FARSLA Antibody [NBP1-88067] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
FARSLA Antibody - BSA Free Western Blot: FARSLA Antibody - BSA Free [NBP1-88067]

Western Blot: FARSLA Antibody - BSA Free [NBP1-88067]

Analysis in human cell line MOLT-4.
FARSLA Antibody - BSA Free Western Blot: FARSLA Antibody - BSA Free [NBP1-88067]

Western Blot: FARSLA Antibody - BSA Free [NBP1-88067]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for FARSLA Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FARSLA

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]

Alternate Names

CML33pheRS, EC 6.1.1.20, FARS, FARSL, FARSLA, FRSA, PheHA, phenylalanine tRNA ligase 1, alpha, cytoplasmic, Phenylalanine--tRNA ligase alpha chain, phenylalanine-tRNA synthetase alpha-subunit, phenylalanine-tRNA synthetase-like, phenylalanine-tRNA synthetase-like, alpha subunit, phenylalanyl-tRNA synthetase alpha chain, phenylalanyl-tRNA synthetase, alpha subunit, phenylalanyl-tRNA synthetase-like, alpha subunit, PheRS

Gene Symbol

FARSA

Additional FARSLA Products

Product Documents for FARSLA Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FARSLA Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FARSLA Antibody - BSA Free

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Protocols

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FAQs

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