FAT1 Antibody (FAT-1 3D7/1) - BSA Free

Novus Biologicals | Catalog # NB100-2693

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Drosophila

Predicted:

Chimpanzee (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # FAT-1 3D7/1

Format

BSA Free
View all FAT1 Primary Antibodies »

Product Specifications

Immunogen

Cytoplasmic domain of drosophila Fat

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Applications for FAT1 Antibody (FAT-1 3D7/1) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
3-8% gradient gels and wet transfer techniques are recommended for the visualisation of FAT1. Positive control(s): Wild type imaginal discs from third instar Drosophila larvae.

Reviewed Applications

Read 1 review rated 4 using NB100-2693 in the following applications:

Formulation, Preparation, and Storage

Purification

Thiophilic Chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAT1

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Alternate Names

FAT tumor suppressor 1, FAT tumor suppressor homolog 1 (Drosophila)

Entrez Gene IDs

2195 (Human)

Gene Symbol

FAT1

Additional FAT1 Products

Product Documents for FAT1 Antibody (FAT-1 3D7/1) - BSA Free

Certificate of Analysis

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Product Specific Notices for FAT1 Antibody (FAT-1 3D7/1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FAT1 Antibody (FAT-1 3D7/1) - BSA Free (1)

4 out of 5
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  • FAT1 Antibody (FAT-1 3D7/1)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: macrophage
    Species: Rat and Human
    Verified Customer | Posted 03/03/2017
    FAT1 WESTERN BLOT LANE 1: RAT CELL LYSATE LANE 2: HUMAN CELL LYSATE
    3-8 PERCENT TRIS ACETATE GEL TRANSFER 10V OVERNIGHT ANTIBODY 1:500 SHOWS HIGHER THAN 500 KDA BAND IN RAT, NOT IN HUMAN SAMPLES.
    FAT1 Antibody (FAT-1 3D7/1) - BSA Free NB100-2693

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