Fatty Acid Synthase/FASN Antibody - BSA Free

Novus Biologicals | Catalog # NB400-114

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Chicken, Feline, Hamster, Plant, Primate

Cited:

Human, Mouse, Rat, Avian - Chicken, Feline, Hamster, Plant

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Fatty Acid Synthase/FASN Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide, conjugated to KLH, made near the N-terminus of mouse FAS. [Swiss-Prot# P19096]

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 24649190). Feline reactivity reported in scientific literature (PMID: 28871635).

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

272 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fatty Acid Synthase/FASN Antibody - BSA Free

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114]

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114]

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114]

Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114]

Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114]

Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114]

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114]

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114]

Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114]

Fatty-Acid-Synthase-FASN-Antibody-Immunocytochemistry-Immunofluorescence-NB400-114-img0012.jpg
Knockdown Validated: Fatty Acid Synthase/FASN Antibody [NB400-114]

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114]

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle. Western blot showing silencing efficiency of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set).
Fatty Acid Synthase/FASN Antibody

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] -

Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed & open symbols indicate replicates from the primary screen & during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- & ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA & 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d & in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC & 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) & ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b & d where representative images are shown & for g where the mean±s.d. is shown for each point of a representative experiment, all data represent the means±s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Fatty Acid Synthase/FASN Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody - BSA Free [NB400-114] -

Fatty acid synthesis requirement for CHIKV life cycle.(a) Impact of FASN or ACLY knockdown on CHIKV replication. Closed and open symbols indicate replicates from the primary screen and during validation, respectively. (b) Western blot showing silencing efficiency of siRNAs used in c. (c) Impact of FASN-, ACC- and ACLY-specific siRNAs on CHIKV replication (n=10 for each data set). (d) Confocal section of CHIKV replicon-infected HeLa cells labelled for FASN, dsRNA and 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 10 μm. (e) Co-localization analysis of cells labelled as in d and in Supplementary Fig. 3b, plotted as Pearson's coefficient per cell. Each symbol corresponds to a cell stack from three independent experiments (n=29 cells for FASN, 30 cells for ACC and 31 cells for ACLY); median values shown in red. (f) Effect of FASN (cerulenin, n=12 for each data set), ACC (TOFA, n=11 for each data set) and ACLY (BMS-303141 n=11 for each data set) inhibitors on CHIKV replication. (g) Real-time cell toxicity assay performed on HeLa cells (n=3 for each point). Excepted for b and d where representative images are shown and for g where the mean+/-s.d. is shown for each point of a representative experiment, all data represent the means+/-s.e.m. of three independent experiments analysed using one-way analysis of variance with Tukey's post test (*P<0.05; **P<0.01; ***P<0.001; NSP≥0.05). NS, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27177310), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Fatty Acid Synthase/FASN Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500immunoprecipitation 1:100

Immunoprecipitation

1:100

Western Blot

1:1000
Application Notes
This Fatty Acid Synthase antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot where a band at ~272 kDa is observed. May see 1 or 2 minor cross-reacting lower MW bands in liver tissue. In ICC/IF cytoplasmic staining can be seen in MCF7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 4 using NB400-114 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fatty Acid Synthase/FASN

Fatty acid synthetase (FASN) is a key player of fatty acid biosynthetic pathway and it catalyzes formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH (de novo lipid synthesis). Localized in cytoplasm and melanosomes, FASN is expressed at low level in normal adult tissues; however, it is significantly upregulated in cancer cells. The FASN expression is regulated by growth factors/growth factor receptors (EGFR and HER2) and steroid hormone /steroid hormone receptors (ER, AR and progesterone receptor). Downstream of the receptors, PI3K-AKT and MAPK signaling mediate FASN expression through SREBP-1c. FASN expression is also controlled by p53 family proteins and SPOT14, lipogenesis-related nuclear protein generally overexpressed in breast cancer. Extracellular stresses including hypoxia and low pH also induce the FASN expression in cancer cells and USP2a stabilises FASN alongwith promotion of its mTOR-dependent selective translational induction. FASN activation provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth/survival advantage possibly acting as a metabolic oncogene. Because FASN-inhibitors are selectively toxic to tumor cells, it has emerged as an attractive target for cancer.

Alternate Names

FASN, OA-519, SDR27X1

Entrez Gene IDs

2194 (Human); 14104 (Mouse)

Gene Symbol

FASN

UniProt

P19096 (Mouse)

Additional Fatty Acid Synthase/FASN Products

Product Documents for Fatty Acid Synthase/FASN Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Fatty Acid Synthase/FASN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fatty Acid Synthase/FASN Antibody - BSA Free

Customer Reviews for Fatty Acid Synthase/FASN Antibody - BSA Free (2)

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  • Name: Kristine Griffett
    Application: Immunofluorescence
    Sample Tested: Mouse Liver, Xenograft tumors
    Species: Mouse
    Verified Customer | Posted 03/12/2013
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Rat
    Species: Rat
    Verified Customer | Posted 12/06/2011

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Protocols

View specific protocols for Fatty Acid Synthase/FASN Antibody - BSA Free (NB400-114):


1. Run ~50 ug of total protein on a 4-15% SDS polyacrylamide gel.
2. Transfer protein to a nitrocellulose membrane.
3. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (~23-27C).
4. Incubate membrane with 1:1000 dilution of NB400-114, diluted in 1XPBS/1% BSA, for 1 hour at room temperature.
5. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
6. Incubate membrane with anti-rabbit IgG-HRP, diluted in 1XPBS/1% BSA, for 30 minutes-1 hour at room temperature.
7. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
8. Detect cross-reacting proteins using a Chemiluminescence Reagent kit: expose ~1 minute.

NOTE: mouse liver lysates were used as a positive control for this antibody.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Fatty Acid Synthase/FASN Antibody - BSA Free

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  • Q: We are interested in your item, NB400-114. Could you please provide us specific protocol of NB400-114 for IHC-P?

    A: If you were requesting the protocol for WB, then we have a special one for this antibody because it is highly acetylated and tightly associated with membrane. However, we don't have any special IHC-P protocol for NB400-114, and the general one can be used: view protocol. (If there was a special IHC-P protocol for this antibody, it would have posted on the webpage for this product; but it does not) The only thing you will concern for using this general IHC-P would be how to do antigen retrieval. Human Protein Atlas shows that for performing antigen retrieval during IHC-P experiments ProteinAtlas site, HIER pH 6 can be applied. For this, both human and mouse tissues are applicable to HIER pH 6.

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