FCF1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04075

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-198 of human FCF1 (NP_057046.1). PCITDCVMAEIEKLGQKYRVALRIAKDPRFERLPCTHKGTYADDCLVQRVTQHKCYIVATVDRDLKRRIRKIPGVPIMYISNHRYNIERMPDDYGAPRF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FCF1 Antibody - Azide and BSA Free

Western Blot: FCF1 AntibodyAzide and BSA Free [NBP3-04075]

Western Blot: FCF1 AntibodyAzide and BSA Free [NBP3-04075]

Western Blot: FCF1 Antibody [NBP3-04075] - Analysis of extracts of 293T cells, using FCF1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]

Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]

Immunocytochemistry/Immunofluorescence: FCF1 Antibody [NBP3-04075] - Analysis of U-2 OS cells using FCF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: FCF1 Antibody - Azide and BSA Free [NBP3-04075]

Immunohistochemistry-Paraffin: FCF1 Antibody - Azide and BSA Free [NBP3-04075]

Immunohistochemistry-Paraffin: FCF1 Antibody [NBP3-04075] - Immunohistochemistry of paraffin-embedded Human esophageal using FCF1 Rabbit pAb (NBP3-04075) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]

Immunocytochemistry/ Immunofluorescence: FCF1 Antibody - Azide and BSA Free [NBP3-04075]

Immunocytochemistry/Immunofluorescence: FCF1 Antibody [NBP3-04075] - Analysis of C6 cells using FCF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for FCF1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FCF1

Essential protein involved in pre-rRNA processing and 40S ribosomal subunit assembly

Alternate Names

Bka, C14orf111, CGI-35, chromosome 14 open reading frame 111, FCF1 small subunit (SSU) processome component homolog (S. cerevisiae), MGC99629, rRNA-processing protein FCF1 homolog

Gene Symbol

FCF1

Additional FCF1 Products

Product Documents for FCF1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FCF1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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