FEM1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77300

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FEM1B Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A. The immunogen is located within the last 50 amino acids of F1A alpha.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FEM1B Antibody - BSA Free

Western Blot: FEM1B AntibodyBSA Free [NBP1-77300]

Western Blot: FEM1B AntibodyBSA Free [NBP1-77300]

Western Blot: FEM1B Antibody [NBP1-77300] - Analysis of FEM1B in mouse (A) and rat (B) liver tissue lysates with anti- FEM1B using this antibody at 1 ug /ml.
Immunohistochemistry-Paraffin: FEM1B Antibody - BSA Free [NBP1-77300]

Immunohistochemistry-Paraffin: FEM1B Antibody - BSA Free [NBP1-77300]

Immunohistochemistry-Paraffin: FEM1B Antibody [NBP1-77300] - Mouse liver tissue with FEM1B antibody at 5 ug/ml.
FEM1B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FEM1B Antibody - BSA Free [NBP1-77300] -

Immunocytochemistry/ Immunofluorescence: FEM1B Antibody - BSA Free [NBP1-77300] - Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 u/mL.
FEM1B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FEM1B Antibody - BSA Free [NBP1-77300] -

Immunocytochemistry/ Immunofluorescence: FEM1B Antibody - BSA Free [NBP1-77300] - Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 ug/ml.

Green: F1A alpha Antibody
Blue: DAPI staining

Applications for FEM1B Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml
Application Notes
An approximately 70 kDa band can be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FEM1B

Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1beta. F1Aalpha/FEM1beta is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.

Alternate Names

DKFZp451E0710, F1AA, F1A-alpha, FEM-1 (C.elegans) homolog b, fem-1 homolog b (C. elegans), FEM1b, FEM1-beta, FEM-1-like death receptor binding protein, Fem-1-like death receptor-binding protein alpha, Fem-1-like in apoptotic pathway protein alpha, FIAA, KIAA0396, protein fem-1 homolog B

Gene Symbol

FEM1B

UniProt

AAF05314 (Human)

Additional FEM1B Products

Product Documents for FEM1B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FEM1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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