FFAR4/GPR120 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-00858

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FFAR4/GPR120 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human GPR120 protein (between residues 200-300) [UniProt Q5NUL3]

Localization

FFAR4/GPR120 is a transmembrane protein with extracellular-helical-cytoplasmic domains

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FFAR4/GPR120 Antibody - BSA Free

Knockout Validated: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

FFAR4-GPR120-Antibody-Knockout-Validated-NBP1-00858-img0010.jpg
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.
Western Blot: FFAR4/GPR120 AntibodyBSA Free [NBP1-00858]

Western Blot: FFAR4/GPR120 AntibodyBSA Free [NBP1-00858]

Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.
Western Blot: FFAR4/GPR120 AntibodyBSA Free [NBP1-00858]

Western Blot: FFAR4/GPR120 AntibodyBSA Free [NBP1-00858]

Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer review.
Immunocytochemistry/ Immunofluorescence: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Immunocytochemistry/ Immunofluorescence: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Immunocytochemistry/Immunofluorescence: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858]

Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.
FFAR4/GPR120 Antibody - BSA Free

Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] -

Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] - The combination of RA & omega -3 PUFAs induces G alpha q-P38 activation through RAR alpha & GPR40(A): Cells were treated with RA(20μM) + EPA(80μM) with or without GPR120-knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (B): Cells were treated with RA(20μM) + EPA(80μM) with or without GPR40-knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (C): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR alpha -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (D): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR beta -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (E): Cells were treated with RA(20μM) + EPA(80μM) with or without RAR gamma -knockdown for 15 min. Cell extracts were prepared & subjected to western blotting analysis. (F): MCF-7 cells were treated with RA(20μM) + EPA(80μM) & their extracts fractionated using an iodixanol density gradient, as described in the Materials & Methods section. Each fraction was subjected to SDS-PAGE & immunoblot analysis using antibodies against the indicated proteins. (G): MCF-7 cells were pretreated with the indicated concentrations of methyl-beta -cyclodextrin (M beta CD) for 1 h, followed by 15 min treatment with RA(20μM) + EPA(80μM). Cell lysates were prepared & subjected to SDS-PAGE & immunoblot analysis. (H): MCF-7 cells were pretreated with the indicated concentrations of methyl-beta -cyclodextrin (M beta CD) for 1 h followed by 24h treatment with RA(20μM) + EPA(80μM), & then subjected to cell counts. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22629), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FFAR4/GPR120 Antibody - BSA Free

Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] -

Western Blot: FFAR4/GPR120 Antibody - BSA Free [NBP1-00858] - The expression of corin & ANP was increased in DHA-induced adipocytes. 3T3-L1 cells were exposed to DHA (100 μM) for 2 d in the presence of the differentiation medium. (A–C) The expression of corin & ANP in DHA-induced adipocytes was analyzed by qRT-PCR & Western blotting. The basal delta-Ct levels for tested genes are presented as Supplementary Table S2. ** p < 0.01. (D,E) The expression of corin & ANP was measured in the GPR120 deficient adipocytes treated with DHA by qRT-PCR & Western blotting. * p < 0.05, ** p < 0.01, *** p < 0.001. (F) 3T3-L1 cells were treated with 1 μM TUG-891, a potent GPR120 agonist for 24 h. The corin & ANP expression levels were analyzed by Western blotting. (G) The concentration of ANP was measured in the media derived from the DHA-induced adipocytes using ELISA. ** p < 0.01. The data are shown as the means ± standard deviations from three or more independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31817347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FFAR4/GPR120 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 34911928)

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NBP1-00858 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FFAR4/GPR120

GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source. They also employ various physiological responses through their GPCRs. One such response occurs when dietary FFAs stimulate GPR120. This stimulation promotes the secretion of Glucagon-like peptide-1 (GLP-1) in vivo and in vitro.GLP-1 belongs to the class of molecules known as the incretins, which are associated with insulin secreted from the pancreas as a result of food intake. GLP-1 also inhibits Glucagon and gastric acid secretion and gastric emptying. Consequently, the role of GPR120 in the secretion of GLP-1 is critical in the treatment of diabetes.

Long Name

Free Fatty Acid Receptor 4

Alternate Names

GPR120, GPR129, GT01, O3FAR1, PGR4

Entrez Gene IDs

338557 (Human); 294075 (Rat)

Gene Symbol

FFAR4

UniProt

Q5NUL3 (Human)

Additional FFAR4/GPR120 Products

Product Documents for FFAR4/GPR120 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FFAR4/GPR120 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FFAR4/GPR120 Antibody - BSA Free

Customer Reviews for FFAR4/GPR120 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: whole cell lysates from mouse bone marrow, spleen, bone marrow derived DCs/macrophages.
    Species: Mouse
    Verified Customer | Posted 04/02/2015
    GPR120 expression in mouse spleen cells, bone marrow, bone marrow derived DC and macrophages
    FFAR4/GPR120 Antibody - BSA Free NBP1-00858

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Protocols

View specific protocols for FFAR4/GPR120 Antibody - BSA Free (NBP1-00858):


Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Western Blot Protocol

1. Perform SDS-PAGE using a 12% gel on samples to be analyzed, loading 25 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute anti-GPR120 primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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