FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

Novus Biologicals | Catalog # H00002246-M02

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Key Product Details

Species Reactivity

Human, Plant

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E12

Format

Azide and BSA Free
View all FGF acidic/FGF1 Primary Antibodies »

Product Specifications

Immunogen

FGF1 (AAH32697, 46 a.a. ~ 155 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Specificity

fibroblast growth factor 1 (acidic)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

Western Blot: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Western Blot: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Western Blot: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of FGF1 expression in transfected 293T cell line by FGF1 monoclonal antibody (M02), clone 2E12.Lane 1: FGF1 transfected lysate(17.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Immunocytochemistry/Immunofluorescence: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of monoclonal antibody to FGF1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Immunohistochemistry-Paraffin: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Immunohistochemistry-Paraffin: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of monoclonal antibody to FGF1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Immunoprecipitation: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Immunoprecipitation: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

Immunoprecipitation: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Analysis of FGF1 transfected lysate using anti-FGF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGF1 MaxPab rabbit polyclonal antibody.
ELISA: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

ELISA: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02]

ELISA: FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - FGF acidic/FGF1 Antibody (2E12) [H00002246-M02] - Detection limit for recombinant GST tagged FGF1 is approximately 10ng/ml as a capture antibody.

Applications for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FGF acidic/FGF1

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described.

Long Name

Fibroblast Growth Factor acidic

Alternate Names

alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1

Entrez Gene IDs

2246 (Human)

Gene Symbol

FGF1

OMIM

131220 (Human)

UniProt

AAH32697 (Human)

Additional FGF acidic/FGF1 Products

Product Documents for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FGF acidic/FGF1 Antibody (2E12) - Azide and BSA Free

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  • Q: What is the heparin binding activity of FGF and VEGF?

    A: These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

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