FGFR1OP Antibody (2B1) - Azide and BSA Free
Novus Biologicals | Catalog # H00011116-M01
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rabbit
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2B1
Format
Azide and BSA Free
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Product Specifications
Immunogen
FGFR1OP (AAH11902, 1 a.a ~ 379 a.a) full length recombinant protein with GST tag.MAATAAAVVAEEDTELRDLLVQTLENSGVLNRIKAELRAAVFLALEEQEKVENKTPLVNESLRKFLNTKDGRLVASLVAEFLQFFNLDFTLAVFQPETSTLQGLEGRENLARDLGIIEAEGTVGGPLLLEVIRRCQQKEKGPTTGEGALDLSDVHSPPKSPEGKTSAQTTPSKKANDEANQSDTSVSLSEPKSKSSLHLLSHETKIGSFLSNRTLDGKDKAGLCPDEDDMEGDSFFDDPIPKPEKTYGLRNEPRK
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28625565). Other species not tested. Rabbit reactivity reported from a verified customer review.
Specificity
FGFR1OP - FGFR1 oncogene partner
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for FGFR1OP Antibody (2B1) - Azide and BSA Free
Western Blot: FGFR1OP Antibody (2B1) [H00011116-M01]
Western Blot: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of FGFR1OP expression in transfected 293T cell line by FGFR1OP monoclonal antibody (M01), clone 2B1. Lane 1: FGFR1OP transfected lysate(43.1 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/ Immunofluorescence: FGFR1OP Antibody (2B1) [H00011116-M01]
Immunocytochemistry/Immunofluorescence: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of monoclonal antibody to FGFR1OP on HeLa cell. Antibody concentration 10 ug/mL.Immunohistochemistry-Frozen: FGFR1OP Antibody (2B1) [H00011116-M01]
Immunohistochemistry-Frozen: FGFR1OP Antibody (2B1) [H00011116-M01] - Rabbit frozen 4% paraformalehyde fixed brain sections. Double labeling with FGFR1OP (Basal bodies in green) and GT335 (cilia in red) antibodies of ependymal whole mount from brain of rabbit kit. IHC-Fr image submitted by a verified customer review.ELISA: FGFR1OP Antibody (2B1) [H00011116-M01]
ELISA: FGFR1OP Antibody (2B1) [H00011116-M01] - Detection limit for recombinant GST tagged FGFR1OP is approximately 0.03 ng/mL as a capture antibody.Applications for FGFR1OP Antibody (2B1) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. It is also useful for immunofluorescence. This FGFR1OP Antibody (2B1) is validated for IHC-Fr from a verified customer review.
Reviewed Applications
Read 1 review rated 4 using H00011116-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: FGFR1OP
Alternate Names
FGFR1 oncogene partner, FOPfibroblast growth factor receptor 1 oncogene partner
Entrez Gene IDs
11116 (Human)
Gene Symbol
CEP43
OMIM
605392 (Human)
UniProt
Additional FGFR1OP Products
Product Documents for FGFR1OP Antibody (2B1) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FGFR1OP Antibody (2B1) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for FGFR1OP Antibody (2B1) - Azide and BSA Free
Customer Reviews for FGFR1OP Antibody (2B1) - Azide and BSA Free (1)
4 out of 5
1 Customer Rating
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Application: Immunohistochemistry-FrozenSample Tested: Rabbit frozen 4% paraformalehyde fixed brain sectionsSpecies: new zealand rabbit and RabbitVerified Customer | Posted 07/01/2020Double labeling with FOFRIOP (Basal bodies in green) and GT335 (cilia in red) antibodies of ependymal whole mount from brain of rabbit kit (d7).
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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