FGFR3 Antibody

Novus Biologicals | Catalog # NB600-1509

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Synthetic peptide: KDLLPPAPPSSGGSRT, corresponding to amino acids 792-806 of the cytoplasmic region of Human FGFR3 with N-terminal added lysine.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Localization

Plasma membrane; Single-pass type I membrane protein

Specificity

Does not react with human FGFR1 or FGFR2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGFR3 Antibody

Western Blot: FGFR3 Antibody [NB600-1509]

Western Blot: FGFR3 Antibody [NB600-1509]

FGFR3-Antibody-Western-Blot-NB600-1509-img0001.jpg
FGFR3 Antibody

Western Blot: FGFR3 Antibody [NB600-1509] -

Western Blot: FGFR3 Antibody [NB600-1509] - (A) Western blot analysis of apoptotic markers following 24 & 48 hrs of selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) treatment. (B) Western blot analysis of autophagic & ER stress markers, evaluated after 1, 3, 6 & 18 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM). (C) Acridine orange staining of cells after 12 hrs of treatment with selenite (5 μM), GS-Se-SG (5 μM) & Se-DL-cystine (100 μM) (magnification 40 ×). (D) High-resolution transmission electron microscopy micrographs of control & 24 hrs Se-DL-cystine-treated cells, showing the presence of autophagosome, as evident from double-layered membrane structure with inclusion bodies. (E) Western blot detection of ubiquitinylated proteins after treatments with different selenium compounds (similar doses as previous) for 6 hrs. Five microgram cell lysate was loaded into each lane. The lane intensity was analysed by using Adobe Photoshop program (version CS5). beta -actin was used as a loading control (Fig. 4A & B). C: Control; 1: selenite; 2: GS-Se-SG; 3: Se-DL-cystine. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24400844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FGFR3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:250

Immunohistochemistry-Paraffin

1:250

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGFR3

Fibroblast growth factors (FGFs) are members of a large family of structurally related polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation in a wide variety of cells of mesodermal, ectodermal and endodermal origin.

Long Name

Fibroblast Growth Factor Receptor 3

Alternate Names

CD333, CEK, FGF R3, JTK4

Entrez Gene IDs

2261 (Human)

Gene Symbol

FGFR3

Additional FGFR3 Products

Product Documents for FGFR3 Antibody

Certificate of Analysis

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Product Specific Notices for FGFR3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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