Fibronectin Antibody (2F4) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80737
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 2F4
Format
Azide and BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human Fibronectin expressed in E. coli. [UniProt# P02751]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
262.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Fibronectin Antibody (2F4) - Azide and BSA Free (NBP2-22113) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fibronectin Antibody (2F4) - Azide and BSA Free
![Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Western-Blot-NBP2-80737-img0003.jpg "Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]")
Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]
Western Blot: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis using Fibronectin mAb against human Fibronectin (AA: 1965-2176) recombinant protein. Expected MW is 49.6 kDa. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80737-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]")
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]
Immunocytochemistry/Immunofluorescence: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis of cultured HepG2 cells using Fibronectin antibody (clone 2F4) at 1:100 dilution. The cells were fixed in formalin and permeabilized using PBS with 0.1% Triton X-100 before incubating them with Fibronectin antibody or the mouse IgG1 isotype control antibody. DyLight 488 conjugated goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody was used at 1: 200 dilution to detect the antigen-primary antibody complexes and the cells were counterstained with DAPI for labeling the nuclei. This Fibronectin antibody generated the expected punctate cytoplasmic-membranous staining and the signal was localized more to the inter-cellular spaces in clustered cells. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80737-img0006.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]")
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis of paraffin-embedded stomach cancer tissues using Fibronectin mouse mAb with DAB staining. Image from the standard format of this antibody.![Flow Cytometry: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80737-img0007.jpg "Flow Cytometry: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]")
Flow Cytometry: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]
Flow Cytometry: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis of HeLa cells using Fibronectin mouse mAb (green) and negative control (red). Image from the standard format of this antibody.![Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Western-Blot-NBP2-80737-img0001.jpg "Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]")
Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]
Western Blot: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis of HeLa (1) and HepG2 (2) whole cell lysates using Fibronectin antibody (2F4) at 2 ug/mL. Image from the standard format of this antibody.![Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Western-Blot-NBP2-80737-img0002.jpg "Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]")
Western Blot: Fibronectin Antibody (2F4)Azide and BSA Free [NBP2-80737]
Western Blot: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis using Fibronectin mAb against HEK293 (1) and Fibronectin (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80737-img0005.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]")
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]
Immunohistochemistry-Paraffin: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Analysis of paraffin-embedded lung cancer tissues using Fibronectin mouse mAb with DAB staining. Image from the standard format of this antibody.![ELISA: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]](https://beta-resources.rndsystems.com/images/products/Fibronectin-Antibody-2F4-Azide-and-BSA-Free-ELISA-NBP2-80737-img0008.jpg "ELISA: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]")
ELISA: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737]
ELISA: Fibronectin Antibody (2F4) - Azide and BSA Free [NBP2-80737] - Red: Control Antigen (100 ng); Purple: Antigen (10 ng); Green: Antigen (50 ng); Blue: Antigen (100 ng). Image from the standard format of this antibody.Applications for Fibronectin Antibody (2F4) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:200 - 1:1000
Western Blot
1:500 - 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (2F4) - Azide and BSA Free
Product Specific Notices for Fibronectin Antibody (2F4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fibronectin Antibody (2F4) - Azide and BSA Free
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Q: What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
A: Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.
