FIP1/RCP Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83600

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FIP1/RCP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AKESKKPESRRSSLLSLMTGKKDVAKGSEGENPLTVPGREKEGMLMGVKPGEDASGPAEDLVRRSEKDTAAVVSRQGSSLNLFEDV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FIP1/RCP Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FIP1/RCP Antibody [NBP1-83600]

Immunocytochemistry/ Immunofluorescence: FIP1/RCP Antibody [NBP1-83600]

Immunocytochemistry/Immunofluorescence: FIP1/RCP Antibody [NBP1-83600] - Staining of human cell line A-431 shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600] - Staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600] - Staining in human small intestine and skeletal muscle tissues.. Corresponding RAB11FIP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600]

Immunohistochemistry-Paraffin: FIP1/RCP Antibody [NBP1-83600] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
FIP1/RCP Antibody - BSA Free Western Blot: FIP1/RCP Antibody - BSA Free [NBP1-83600]

Western Blot: FIP1/RCP Antibody - BSA Free [NBP1-83600]

Analysis in human cell lines A-431 and U-251MG using Anti-RAB11FIP1 antibody. Corresponding RAB11FIP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for FIP1/RCP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FIP1/RCP

Proteins of the large Rab GTPase family have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001)

Alternate Names

DKFZp686E2214, EC 3.2.1.28, EC 6.1.1.7, FLJ22524, FLJ22622, MGC78448, NOEL1A, RAB11 coupling protein, RAB11 family interacting protein 1 (class I), rab11 family-interacting protein 1, rab11-FIP1, Rab-coupling protein, Rab-interacting recycling protein, RCPRab effector protein

Gene Symbol

RAB11FIP1

Additional FIP1/RCP Products

Product Documents for FIP1/RCP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FIP1/RCP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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