Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [FITC] - Chimeric

Novus Biologicals | Catalog # NBP2-52666F

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Neutralization, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # D1-4G2-4-15 (4G2)
View all Flavivirus group antigen Primary Antibodies »

Product Specifications

Immunogen

This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric was prepared from Dengue Virus type 2 antigens.

Reactivity Notes

Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae

Specificity

This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [FITC] [NBP2-52666F] - Left: Uninfected cells. Right: Cells infected with yellow fever 17D virus, MOI=1. Image from verified customer review.

Applications

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-52666F in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Flavivirus group antigen

Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).

The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).

Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.

References

1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142

2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302

3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338

4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180

5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33

6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004

Alternate Names

Envelope Protein, Protein E

Additional Flavivirus group antigen Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews (1)

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  • Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [FITC]
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Monocyte-derived immature dendritic cells, MoDC infected with yellow fever 17D and Cells infected with yellow fever 17D virus
    Species: Human
    Verified Customer | Posted 04/25/2019
    Left: Uninfected cells Right: Cells infected with yellow fever 17D virus, MOI=1
    Cells were infected with YF17D for 36h, MOI =1. Antibody dilution = 1:100 This antibody didn't work with IC Fixation + IC permeabilisation buffer (eBioscience). It works well with BD Cytofix + homemade perm buffer (0.5% Saponin, 1% BSA and 0.01 Sodium azide.
    Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) [FITC] - Chimeric NBP2-52666F

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