Flotillin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-30881

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Block/Neutralize

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Flotillin 2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary.

Localization

Cell membrane, Peripheral membrane protein, Membrane, caveola, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Flotillin 2 Antibody - BSA Free

Western Blot: Flotillin 2 Antibody [NBP1-30881]

Western Blot: Flotillin 2 Antibody [NBP1-30881]

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP1-30881]

Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody [NBP1-30881]

Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881]

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881]

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Flotillin 2 Antibody [NBP1-30881]

Western Blot: Flotillin 2 Antibody [NBP1-30881]

Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000.
Western Blot: Flotillin 2 Antibody [NBP1-30881]

Western Blot: Flotillin 2 Antibody [NBP1-30881]

Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881]

Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881]

Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881] - Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 ug of Flotillin 2 antibody [C3], C-term. Western blot analysis was performed using Flotillin 2 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Flotillin 2 Antibody

Western Blot: Flotillin 2 Antibody [NBP1-30881] -

Western Blot: Flotillin 2 Antibody [NBP1-30881] -Flotillin 2 antibody [C3], C-term detects Flotillin 2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody [C3], C-term diluted by 1:1000.

Applications for Flotillin 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-30881 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Flotillin 2

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling.

Alternate Names

Epidermal surface antigen, ESA1Membrane component chromosome 17 surface marker 1, ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1), flotillin 2

Entrez Gene IDs

2319 (Human)

Gene Symbol

FLOT2

UniProt

Q14254 (Human)

Additional Flotillin 2 Products

Product Documents for Flotillin 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Flotillin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Flotillin 2 Antibody - BSA Free

Customer Reviews for Flotillin 2 Antibody - BSA Free (1)

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  • Flotillin 2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 10/11/2019
    Flotillin 2 Antibody - BSA Free NBP1-30881

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Protocols

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