Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

Novus Biologicals | Catalog # NB110-94155

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # 12B6

Format

Azide and BSA Free
View all Flt-3/Flk-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse Flt-3/Flk-2/CD135 (extracellular domain)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free [NB110-94155]

Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free [NB110-94155]

Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) [NB110-94155] - Flt-3 antibody staining in mouse lung cancer tissue. IHC-P image submitted by a verified customer review.
Immunohistochemistry: Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free [NB110-94155]

Immunohistochemistry: Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free [NB110-94155]

Immunohistochemistry: Flt3/CD135 Antibody (12B6) [NB110-94155] - Paraffin kidney and lung sections from LPS treated mice were subjected to IHC using anti Flt3/CD135 Antibody (12B6) RM0021-12B6

Applications for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1:100-1:1000

Reviewed Applications

Read 1 review rated 5 using NB110-94155 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Flt-3/Flk-2

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Long Name

fms-like Tyrosine Kinase 3

Alternate Names

CD135, Flk-2, Flt3, STK-1

Entrez Gene IDs

2322 (Human)

Gene Symbol

FLT3

Additional Flt-3/Flk-2 Products

Product Documents for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free (1)

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Customer Images

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  • Flt-3/Flk-2/CD135 Antibody (12B6)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Lung cancer tissue
    Species: Mouse
    Verified Customer | Posted 11/30/2021
    Flt-3 Antibody staining in lung cancer tissue
    4% formaldehyde fixed paraffin sections were processed in dilutions of ethanol. 1ry antibody dilution was 1:100, 2ry antibody 1:400.
    Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free NB110-94155

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