Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80739
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Lambda Clone # 7E8.2C8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Partial recombinant human FLT3 protein (between amino acids 250-500) [UniProt P36888]
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: ~86% to Mouse and Rat's FLT3 protein.
Localization
Endoplasmic reticulum lumen, Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Lambda
Scientific Data Images for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
![Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8)Azide and BSA Free [NBP2-80739]](https://resources.rndsystems.com/images/products/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Azide-and-BSA-Free-Western-Blot-NBP2-80739-img0001.jpg "Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8)Azide and BSA Free [NBP2-80739]")
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8)Azide and BSA Free [NBP2-80739]
Western Blot: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - WB analysis of lysates from (A) Human Embryonic Kidney 293 / HEK293 and (B) Human Embryonic carcinoma NCCIT cell lines using Flt-3/Flk-2/CD135 antibody (clone 7E8.2C8) at 2ug/ml concentration. The antibody detected single specific bands in both samples at the expected molecular weight position. Image from the standard format of this antibody.![Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]](https://resources.rndsystems.com/images/products/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80739-img0002.jpg "Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]")
Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]
Immunocytochemistry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver cancer using Flt-3/Flk-2/CD135 antibody (clone 7E2.2C8) at 15 ug/ml concentration. The antibody generated specific cytoplasmic immunostaining of FLT3 in the cancerous cells w![Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]](https://resources.rndsystems.com/images/products/Flt-3-Flk-2-CD135-Antibody-7E8-2C8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80739-img0003.jpg "Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]")
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739]
Flow Cytometry: Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free [NBP2-80739] - HEK293 cells were stained (surface) with Flt-3/Flk-2/CD135 antibody (clone: 7E8.2C8; red) or isotype control (mouse IgG2 lambda; blue) and positive staining observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells (PPI negative) were gated for analysis Image from the standard format of this antibody.Applications for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
2 ug/million cells
Immunohistochemistry
5-15 ug/ml
Immunohistochemistry-Paraffin
5-15 ug/ml
Western Blot
2 ug/ml
Application Notes
FLT3 is a 993 amino acids long protein with predicted molecular weight of 112.9 kDa, however, it undergoes extensive glycosylation and phosphorylation so that the mature protein will have relatively higher molecular weights. In WB assay, the glycosylated protein species may run between 113-160 kDa position, while the phosphorylated protein may result on generation of a doublet band.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Flt-3/Flk-2
Long Name
fms-like Tyrosine Kinase 3
Alternate Names
CD135, Flk-2, Flt3, STK-1
Gene Symbol
FLT3
Additional Flt-3/Flk-2 Products
Product Documents for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
