Key Product Details

Species Reactivity

Non-species specific

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Dot Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG
View all Fluorescein Primary Antibodies »

Product Specifications

Immunogen

Fluorescein conjugated to Goat IgG

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum and Fluorescein conjugated IgG.

Scientific Data Images for Fluorescein Antibody [HRP]

Dot Blot: Fluorescein Antibody [HRP] [NB120-6656]
Fluorescein Antibody [HRP]

Fluorescein Antibody [HRP]

Dot Blot Results of Fluorescein Antibody [HRP]. Blot row top (T): FITC-BSA conjugated. Blot row bottom (B): BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Anti-Fluorescein Antibody HRP at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, DyLight 549 filter.

Applications for Fluorescein Antibody [HRP]

Application
Recommended Usage

ELISA

1:200000

Immunohistochemistry

1:500-1:2500

Immunohistochemistry-Paraffin

1:500-1:2500

Western Blot

1:5000-1:10000
Application Notes
This product has been tested by ELISA, dot blot, and western blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and in situ hybridization, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fluorescein

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Alternate Names

FITC, fluorescein isothyocianate

Additional Fluorescein Products

Product Documents for Fluorescein Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluorescein Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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