Key Product Details

Species Reactivity

Non-species specific

Applications

Western Blot, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence

Label

Rhodamine (Excitation = 570 nm, Emission = 590 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Fluorescein conjugated to Goat IgG

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Red Excitation Wavelength: 550 Emission Wavelength: 570
Conjugation Chemistry: Isothiocyanate

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA

Applications for Fluorescein Antibody [Rhodamine] - F(ab')2

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

>1:20000

Immunocytochemistry/ Immunofluorescence

1:500-1:2500

Western Blot

>1:10000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fluorescein

Alternate Names

FITC, fluorescein isothyocianate

Additional Fluorescein Products

Product Documents for Fluorescein Antibody [Rhodamine] - F(ab')2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluorescein Antibody [Rhodamine] - F(ab')2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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