Fluorescein Antibody [Rhodamine] - F(ab')2
Novus Biologicals | Catalog # NBP1-97384
Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence
Label
Rhodamine (Excitation = 570 nm, Emission = 590 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Fluorescein conjugated to Goat IgG
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Red Excitation Wavelength: 550 Emission Wavelength: 570
Conjugation Chemistry: Isothiocyanate
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Red Excitation Wavelength: 550 Emission Wavelength: 570
Conjugation Chemistry: Isothiocyanate
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA
Applications for Fluorescein Antibody [Rhodamine] - F(ab')2
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
>1:20000
Immunocytochemistry/ Immunofluorescence
1:500-1:2500
Western Blot
>1:10000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: Fluorescein
Alternate Names
FITC, fluorescein isothyocianate
Additional Fluorescein Products
Product Documents for Fluorescein Antibody [Rhodamine] - F(ab')2
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Fluorescein Antibody [Rhodamine] - F(ab')2
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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