Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen

Label

Texas Red (Excitation = 589 nm, Emission = 615 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Fluorescein conjugated to Goat IgG

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

Fluorescein Antibody Texas Red(TM) Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin.

Applications for Fluorescein Antibody [Texas Red]

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

>1:20000

Immunocytochemistry/ Immunofluorescence

>1:20000

Western Blot

>1:10000
Application Notes
This product is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:19667884)

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fluorescein

Alternate Names

FITC, fluorescein isothyocianate

Additional Fluorescein Products

Product Documents for Fluorescein Antibody [Texas Red]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluorescein Antibody [Texas Red]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fluorescein Antibody [Texas Red]

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Protocols

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FAQs

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