FOXO3 Antibody (1E2) - BSA Free

Name: FOXO3 Antibody (1E2) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26571
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26571

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E2 expressed in HEK293

Format

BSA Free

View all FoxO3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human FOXO3 [UniProt O43524]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FOXO3 Antibody (1E2) - BSA Free

Immunohistochemistry: FOXO3 Antibody (1E2) [NBP3-26571] -

Immunohistochemistry: FOXO3 Antibody (1E2) [NBP3-26571] - Image of FOXO3 Antibody (1E2) diluted at 1:180 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry: FOXO3 Antibody (1E2) [NBP3-26571] -

Western Blot: FOXO3 Antibody (1E2) [NBP3-26571] -

Western Blot: FOXO3 Antibody (1E2) [NBP3-26571] - Positive Western Blot detected in: 293 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate.
All lanes: FOXO3 Antibody at 1.8ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 72, 49 KDa
Observed band size: 72-90 KDa

Immunocytochemistry/Immunofluorescence: FOXO3 Antibody (1E2) [NBP3-26571] -

Immunocytochemistry/Immunofluorescence: FOXO3 Antibody (1E2) [NBP3-26571] - Staining of PC3 cells with FOXO3 Antibody (1E2) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

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Applications for FOXO3 Antibody (1E2) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: FoxO3

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Long Name

Forkhead Box O3

Alternate Names

AF6q21, FKHRL1, FKHRL1P2, FOXO3A

Gene Symbol

FOXO3

Additional FoxO3 Products

Product Documents for FOXO3 Antibody (1E2) - BSA Free

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Certificate of Analysis

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Product Specific Notices for FOXO3 Antibody (1E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.