Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Flow Cytometry, Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Single Cell Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FoxP3 Antibody - BSA Free (NB100-39002) is a polyclonal antibody validated for use in IHC, WB, Flow, Dual RNAscope ISH-IHC, ICC/IF and IP. Anti-FoxP3 Antibody: Cited in 61 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FoxP3 Antibody - BSA Free

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0025.jpg
Immunohistochemistry: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry: FoxP3 Antibody - BSA Free [NB100-39002]

FoxP3-Antibody-Immunohistochemistry-NB100-39002-img0027.jpg
Flow Cytometry: FoxP3 Antibody - BSA Free [NB100-39002]

Flow Cytometry: FoxP3 Antibody - BSA Free [NB100-39002]

Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.
Immunohistochemistry: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE bovine spleen tissue. Deparaffinization and antigen retrieval was carried out with PTlink from Dako, citrate buffer pH 6. Anti-Foxp3 antibody was used at a 1:100 dilution. Secondary reagent: Envision, from Dako. Staining: DAB and hematoxylin. IHC-P image submitted by a verified customer review.
Western Blot: FoxP3 AntibodyBSA Free [NB100-39002]

Western Blot: FoxP3 AntibodyBSA Free [NB100-39002]

Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution).
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002]

Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002]

Immunocytochemistry/Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.
Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody - BSA Free [NB100-39002]

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counterstained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.
Flow Cytometry: FoxP3 Antibody - BSA Free [NB100-39002]

Flow Cytometry: FoxP3 Antibody - BSA Free [NB100-39002]

Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown.
Flow Cytometry: FoxP3 Antibody - BSA Free [NB100-39002]

Flow Cytometry: FoxP3 Antibody - BSA Free [NB100-39002]

Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD8A and FoxP3 antibody at 1:400 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CD4 and FOXP3 antibody at 1:200 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).
FoxP3 Antibody - BSA Free

Immunohistochemistry: FoxP3 Antibody - BSA Free [NB100-39002] -

Effects of anaphylatoxins on transcription factor phosphorylation in ARPE-19 cells.(D) Immunofluorescence staining of FoxP3. Upper panel: Lewis rat (albino) eye with experimental uveitis; lower panel: normal rat eye. Asterisks mark immune cells infiltrating the retina from the choroid through the retinal pigment epithelium (RPE) in the eye with uveitis (clinical score of 2, histological score of 1).
FoxP3 Antibody - BSA Free

FoxP3 in MCF7 Human Cell Line -

FoxP3 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-FoxP3 Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB100-39002G) (green) at 10 µg/mL overnight at 4C. Cells were stained counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
FoxP3 Antibody - BSA Free

FoxP3 in MCF7 Human Cell Line.

FoxP3 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-FoxP3 Affinity Purified Polyclonal Antibody conjugated to DyLight 550 (Catalog # NB100-39002R) (red) at 10 µg/mL overnight at 4C. Cells were stained counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.
FoxP3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002] -

Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002] - CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. (A, B) Representative flow cytometry dot plots (A) & percentage (B) of CD4+CD25+FOXP3+ cells among the CD4+ T cells in the spinal cord after sham or SCI surgery. Cells were gated on 7-AAD negative population & the numbers inside plots refer to % Treg cells (n=5). (C, D) Representative histogram of CCR10 (C) & CCR3 (D) expression in CD4+CD25+FOXP3+ cells as gated in (A). (E) CD4+CD25+FOXP3+ cells in the spinal cord from sham & SCI mice were sorted out & analyzed by Western blotting to detect the expression of CCR10 & CCR3. GAPDH was used as a loading control. (F) Double immunostaining of FOXP3 (red) & CCR10 (green) in the spinal cord sections after sham or SCI surgery. The merged images are also shown. Arrows indicate cells with positive staining & colocalization. Scale bar, 100 μm. Data are mean ± SD. The statistical analysis was performed using Student’s t-test. **, P<0.01. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31557129), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FoxP3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002] -

Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody - BSA Free [NB100-39002] - Effects of anaphylatoxins on transcription factor phosphorylation in ARPE-19 cells. (A–C) Protein phosphorylation of (A) CREB (Ser133); (B) FoxP3 (Ser418); & (C) FOXO1 (Ser256) was examined in ARPE-19 cells treated with C3a, C5a, or the combination of C3a/C5a at 1, 5, 10, & 15 min. Phosphorylation levels were normalized to beta -actin (relative p-protein levels), & slopes of the time course were determined. Anaphylatoxins were applied at concentrations of 300 nM (C3a) & 50 nM (C5a). Data are mean + SEM, n = 3, *p < 0.05, ***p < 0.001 (Student’s t-test). (D) Immunofluorescence staining of FoxP3. Upper panel: Lewis rat (albino) eye with experimental uveitis; lower panel: normal rat eye. Asterisks mark immune cells infiltrating the retina from the choroid through the retinal pigment epithelium (RPE) in the eye with uveitis (clinical score of 2, histological score of 1). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fimmu.2017.00703/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FoxP3 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:8000

Immunoblotting

reported in scientific literature (PMID 26485285)

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400. Use reported by customer review

Single Cell Western

100 ug/mL

Western Blot

1:2000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. SCW is validating using FoxP3-transfected HEK293T cells.

Reviewed Applications

Read 2 reviews rated 4 using NB100-39002 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Epitope affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FoxP3

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID, anti-human foxp3, anti-mouse foxp3, foxp3 ihc, foxp3 paraffin

Entrez Gene IDs

20371 (Mouse)

Gene Symbol

FOXP3

UniProt

Q99JB6 (Mouse)

Additional FoxP3 Products

Product Documents for FoxP3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FoxP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FoxP3 Antibody - BSA Free

Customer Reviews for FoxP3 Antibody - BSA Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • FoxP3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE spleen tissue
    Species: Bovine, Sheep and Goat
    Verified Customer | Posted 10/21/2019
    FFPE bovine spleen tissue
    Epitope retrieval: PTlink from Dako with pH6 citrate buffer. Primary antibody dilution: 1:100 DAB staining and hematoxylin contrast.
    FoxP3 Antibody - BSA Free NB100-39002
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse Spleen
    Species: Mouse
    Verified Customer | Posted 10/06/2015
    Mouse spleen, cells in white pulp
    FoxP3 Antibody - BSA Free NB100-39002

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Protocols

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FAQs for FoxP3 Antibody - BSA Free

Showing  1 - 5 of 5 FAQs Showing All

    • A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

    • Q: What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
      A: For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site

    • Q: What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
      A: The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.

    • A: A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

    • A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
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