FUS Antibody (CL0190) - BSA Free

Knockdown Validated: FUS Antibody (CL0190) [NBP2-52874]

Knockdown Validated: FUS Antibody (CL0190) [NBP2-52874] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-FUS monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.

Western Blot: FUS Antibody (CL0190) [NBP2-52874]

Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction, Lane 3: Human cell line HeLa membrane fraction, Lane 4: Human cell line HeLa nuclear fraction, Lane 5: Human cell line HeLa chromatin fraction, Lane 6: Human cell line HeLa cytos

Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874]

Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U2OS cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining shown in green.

Western Blot: FUS Antibody (CL0190) [NBP2-52874]

Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa]Lane 2: Human cell line HeLaLane 3: Human cell line A-431Lane 4: Human cell line MCF-7Lane 5: Human cell line U2-OSLane 6: Human cell line Hep-G2

Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874]

Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in A431 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874]

Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in HeLa cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874]

Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in MCF7 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874]

Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U251 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874]

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874]

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874]

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874]

Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human liver shows only very weak nuclear positivity in hepatocytes.

Western Blot: FUS Antibody (CL0190) - BSA Free [NBP2-52874] -

FUS antibody screening by Western Blot.Lysates of HeLa (WT andFUS KO) were prepared and 30 μg of protein were processed for Western Blot with the indicated FUS antibodies. The Ponceau stained transfers of each blot are presented to show equal loading of WT and KO lysates and protein transfer efficiency from the acrylamide gels to the nitrocellulose membrane. Antibody dilutions were chosen according to the recommendations of the antibody supplier. An exception was given for antibody GTX101810, which was titrated to 1/3000, as the signal was too weak when following the supplier’s recommendation. Antibody dilution used: NBP2-52874* at 1/1000; GTX101810 at 1/3000; GTX01039* at 1/1000; 60160-1-Ig* at 1/10000; 11570-1-AP at 1/4000; MA3-089* at 1/2000; MA5-32483** at 1/1000, ab124923** at 1/5000; ab154141* at 1/1000; ab243880** at 1/1000. Predicted band size: 53 kDa. Observed specific band size: ~70 kDa. *Monoclonal antibody; **Recombinant antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37384305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunoprecipitation: FUS Antibody (CL0190) - BSA Free [NBP2-52874] -

FUS antibody screening by immunoprecipitation.HeLa lysates were prepared, and IP was performed using 1.0 μg of the indicated FUS antibodies pre-coupled to protein G or protein A Sepharose beads. Samples were washed and processed for Western Blot with the indicated FUS antibody. For Western Blot, NBP2-52874* and ab243880** were used at a dilution of 1/2000. The Ponceau stained transfers of each blot are shown for similar reasons as inFigure 1. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. *Monoclonal antibody; **Recombinant antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37384305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.