FUS Antibody (CL0190) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44055
![FUS Antibody (CL0190) - Azide and BSA Free Western Blot: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512182775.jpg "Western Blot: FUS Antibody [NBP3-44055]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0190
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P35637, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for FUS Antibody (CL0190) - Azide and BSA Free
Western Blot: FUS Antibody [NBP3-44055]
Lane 1: Marker [kDa]Lane 2: Human cell line HeLa cytoplasmic fraction
Lane 3: Human cell line HeLa membrane fraction
Lane 4: Human cell line HeLa nuclear fraction
Lane 5: Human cell line HeLa chromatin fraction
Lane 6: Human cell line HeLa cytoskeletal fraction
![FUS Antibody (CL0190) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512163663.jpg "Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]")
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]
Staining in HeLa cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![FUS Antibody (CL0190) - Azide and BSA Free Western Blot: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512145354.jpg "Western Blot: FUS Antibody [NBP3-44055]")
Western Blot: FUS Antibody [NBP3-44055]
Lane 1: Marker [kDa]Lane 2: Human cell line HeLa
Lane 3: Human cell line A-431
Lane 4: Human cell line MCF-7
Lane 5: Human cell line U2-OS
Lane 6: Human cell line Hep-G2
![FUS Antibody (CL0190) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512111814.jpg "Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]")
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]
Staining in MCF7 cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![FUS Antibody (CL0190) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-28220251273777.jpg "Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]")
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]
Staining in U2OS cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![FUS Antibody (CL0190) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512163610.jpg "Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]")
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]
Staining in U251 cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![FUS Antibody (CL0190) - Azide and BSA Free Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-28220251210425.jpg "Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]")
![FUS Antibody (CL0190) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512173128.jpg "Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]")
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NBP3-44055]
Staining in A431 cell line with Anti-FUS monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).![FUS Antibody (CL0190) - Azide and BSA Free Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512332966.jpg "Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]")
Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]
Staining of human cerebral cortex shows strong nuclear positivity in neurons.![FUS Antibody (CL0190) - Azide and BSA Free Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512405585.jpg "Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]")
Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]
Staining of human endometrium shows strong nuclear positivity in glandular cells.![FUS Antibody (CL0190) - Azide and BSA Free Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202512495817.jpg "Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]")
Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]
Staining of human liver shows only very weak nuclear positivity in hepatocytes.![FUS Antibody (CL0190) - Azide and BSA Free Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202513381593.jpg "Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]")
Immunohistochemistry-Paraffin: FUS Antibody [NBP3-44055]
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.![FUS Antibody (CL0190) - Azide and BSA Free Western Blot: FUS Antibody [NBP3-44055]](https://resources.rndsystems.com/images/products/nbp3-44055_mouse-fus-mab-cl0190-azide-and-bsa-free-282202513381514.jpg "Western Blot: FUS Antibody [NBP3-44055]")
Applications for FUS Antibody (CL0190) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: FUS
Long Name
FUS RNA binding protein
Alternate Names
ALS6, altFUS, ETM4, FUS1, HNRNPP2, POMP75, TLS
Gene Symbol
FUS
Additional FUS Products
Product Documents for FUS Antibody (CL0190) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FUS Antibody (CL0190) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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