Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FUS Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26403203).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Knockout (KO) Validated Rabbit FUS Antibody - BSA Free (NB100-565) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-FUS Antibody: Cited in 31 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FUS Antibody - BSA Free

Knockout Validated: FUS Antibody [NB100-565]

Western Blot: FUS Antibody [NB100-565]

Western Blot: FUS Antibody [NB100-565] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-565) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.
Western Blot: FUS Antibody [NB100-565]

Western Blot: FUS Antibody [NB100-565]

FUS-Antibody-Western-Blot-NB100-565-img0023.jpg
Immunocytochemistry/ Immunofluorescence: FUS Antibody [NB100-565]

Immunocytochemistry/ Immunofluorescence: FUS Antibody [NB100-565]

Immunocytochemistry/Immunofluorescence: FUS Antibody [NB100-565] - FUS (NB100-565) (green), b-III Tubulin (white), DAPI (blue). FUS antibody dilution: 1:300 in PBST (0.1% Triton X-100) + 10% GS O/N 4C. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FUS Antibody [NB100-565]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-565]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of mouse renal cells carcinoma. Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1 ug/mL). Detection: DA
Western Blot: FUS Antibody [NB100-565]

Western Blot: FUS Antibody [NB100-565]

Western Blot: FUS Antibody [NB100-565] - Whole cell lysate (50 ug) from HepG2, HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-FUS antibody used for WB at 0.04 ug/mL. Detection: chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: FUS Antibody [NB100-565]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-565]

Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1 ug/mL). Detection: DAB
Immunoprecipitation: FUS Antibody [NB100-565]

Immunoprecipitation: FUS Antibody [NB100-565]

Immunoprecipitation: FUS Antibody [NB100-565] - Detection of human FUS by Western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-565 used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (NBP2-76415) and rabbit anti-FUS antibody NB100-561. For blotting immunoprecipitated FUS, NB100-565 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.
FUS Antibody

Immunocytochemistry/ Immunofluorescence: FUS Antibody [NB100-565] -

Immunocytochemistry/ Immunofluorescence: FUS Antibody [NB100-565] - hnRNP R & hnRNP Q form frequent inclusions in FTLD-FUS. Representative images of FUS, TRN1, hnRNP R & hnRNP Q immunohistochemical staining in the granule cell layer of the dentate fascia of the hippocampus in NIFID & aFTLD-U subtypes of FTLD-FUS. Single arrows indicate neuronal cytoplasmic inclusions & double arrows highlight intranuclear inclusions. Scale bars represent 50 μm in all images Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30755280), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FUS Antibody

Immunocytochemistry/ Immunofluorescence: FUS Antibody [NB100-565] -

Immunocytochemistry/ Immunofluorescence: FUS Antibody [NB100-565] - hnRNP R co-localises with FUS inclusions in NIFID & aFTLD-U cases. Representative images of double-label immunofluorescence in the cortex (a & b) & granular cell layer of the dentate gyrus (c & d) of a NIFID & aFTLD-U case demonstrating colocalisation of FUS (green) & hnRNP R (red) in neuronal cytoplasmic inclusions (white arrows) & intranuclear neuronal inclusions (white arrow heads). Neuronal nuclei are counterstained with DAPI. Scale bars represent 20 μm in all images Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30755280), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FUS Antibody

Western Blot: FUS Antibody [NB100-565] -

Western Blot: FUS Antibody [NB100-565] - Expression of EGFP‐FUS reduces the expression of endogenous FUSHEK293 cells were transfected with control EGFP, EGFP‐FUS, EGFP‐FUSR521C or EGFP‐FUSR518K & 72 h post‐transfection, the samples were probed on immunoblots for FUS (using FUS antibody) & tubulin as a loading control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27418313), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FUS Antibody

Western Blot: FUS Antibody [NB100-565] -

Western Blot: FUS Antibody [NB100-565] - FUS does not bind VAPB or PTPIP51 in immunoprecipitation assays from transfected HEK293 cellsCells were transfected as indicated with control vector (CTRL), HA‐FUS + CTRL, myc‐VAPB + CTRL, or myc‐VAPB + either HA‐FUS, HA‐FUSR521C or HA‐FUSR518K. VAPB was immunoprecipitated via the myc‐tag & the samples probed on immunoblots for VAPB using rabbit VAPB antibody & for co‐immunoprecipitating FUS via the HA tag. Input VAPB & FUS were detected using myc & HA antibodies.Cells were transfected as indicated with control vector (CTRL), HA‐FUS + CTRL, HA‐PTPIP51 + CTRL or HA‐PTPIP51 + either HA‐FUS, HA‐FUSR521C or HA‐FUSR518K. PTPIP51 was immunoprecipitated using rat anti‐PTPIP51 & the samples probed for PTPIP51 using rabbit anti‐HA antibody & for co‐immunoprecipitating FUS using rabbit FUS antibody. Input PTPIP51 & FUS were detected using PTPIP51 & EGFP antibodies, respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27418313), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FUS Antibody

Western Blot: FUS Antibody [NB100-565] -

Western Blot: FUS Antibody [NB100-565] - FUS does not bind VAPB or PTPIP51 in immunoprecipitation assays from transfected HEK293 cellsCells were transfected as indicated with control vector (CTRL), HA‐FUS + CTRL, myc‐VAPB + CTRL, or myc‐VAPB + either HA‐FUS, HA‐FUSR521C or HA‐FUSR518K. VAPB was immunoprecipitated via the myc‐tag & the samples probed on immunoblots for VAPB using rabbit VAPB antibody & for co‐immunoprecipitating FUS via the HA tag. Input VAPB & FUS were detected using myc & HA antibodies.Cells were transfected as indicated with control vector (CTRL), HA‐FUS + CTRL, HA‐PTPIP51 + CTRL or HA‐PTPIP51 + either HA‐FUS, HA‐FUSR521C or HA‐FUSR518K. PTPIP51 was immunoprecipitated using rat anti‐PTPIP51 & the samples probed for PTPIP51 using rabbit anti‐HA antibody & for co‐immunoprecipitating FUS using rabbit FUS antibody. Input PTPIP51 & FUS were detected using PTPIP51 & EGFP antibodies, respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27418313), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FUS Antibody

Western Blot: FUS Antibody [NB100-565] -

Western Blot: FUS Antibody [NB100-565] - FUS does not bind VAPB or PTPIP51 in immunoprecipitation assays from transfected HEK293 cellsCells were transfected as indicated with control vector (CTRL), HA‐FUS + CTRL, myc‐VAPB + CTRL, or myc‐VAPB + either HA‐FUS, HA‐FUSR521C or HA‐FUSR518K. VAPB was immunoprecipitated via the myc‐tag & the samples probed on immunoblots for VAPB using rabbit VAPB antibody & for co‐immunoprecipitating FUS via the HA tag. Input VAPB & FUS were detected using myc & HA antibodies.Cells were transfected as indicated with control vector (CTRL), HA‐FUS + CTRL, HA‐PTPIP51 + CTRL or HA‐PTPIP51 + either HA‐FUS, HA‐FUSR521C or HA‐FUSR518K. PTPIP51 was immunoprecipitated using rat anti‐PTPIP51 & the samples probed for PTPIP51 using rabbit anti‐HA antibody & for co‐immunoprecipitating FUS using rabbit FUS antibody. Input PTPIP51 & FUS were detected using PTPIP51 & EGFP antibodies, respectively. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27418313), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
FUS Antibody

Western Blot: FUS Antibody [NB100-565] -

Western Blot: FUS Antibody [NB100-565] - Expression of wild‐type & ALS/FTD‐mutant FUS reduces ER–mitochondria associations in NSC34 cellsAExpression of FUS does not alter expression of VAPB, PTPIP51 or mitofusin‐2 (MFN2) in transfected NSC34 cells. Immunoblots of NSC34 cells transfected with EGFP as a control (CTRL), or wild‐type or mutant EGFP‐FUS. Transfected cells were purified via EGFP using a cell sorter & the samples probed on immunoblots as indicated. On the FUS immunoblot, samples were probed with FUS antibody to show endogenous & transfected proteins; tubulin is shown as a loading control.BRepresentative electron micrographs of ER–mitochondria associations in NSC34 cells transfected with control EGFP vector (CTRL), EGFP‐FUS, EGFP‐FUSR521C or EGFP‐FUSR518K as indicated; arrowheads with loops show regions of association. Scale bar = 200 nm. Bar chart shows % of the mitochondrial surface closely apposed to ER in the different samples. Data were analysed by one‐way analysis of variance (ANOVA) followed by Tukey's multiple comparison test. N = 27–30 cells & 247–424 mitochondria, error bars are s.e.m.; ***P < 0.001.C, DsiRNA loss of FUS does not affect ER–mitochondria associations or alter expression of VAPB, PTPIP51 or mitofusin‐2 (MFN2) in NSC34 cells. (C) Immunoblots of cells either mock transfected or treated with control (CTRL) or FUS siRNAs; GAPDH is shown as a loading control. (D) Representative electron micrographs of ER–mitochondria associations in control (CTRL) & FUS siRNA‐treated cells. Arrowheads with loops show regions of association. Scale bar = 200 nm. Data analysed by unpaired t‐test. N = 27–28 cells & 193–202 mitochondria, error bars are s.e.m. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27418313), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FUS Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2000

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in scientific literature (PMID: 28444573). FUS antibody validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 4 reviews rated 5 using NB100-565 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FUS

FUS is a nucleic acid-binding protein whose N-terminal half functions as a transcriptional activator domain in fusion oncoproteins found in human leukemias and liposarcomas. FUS has been reported to be associated with the nuclear matrix and has been implicated in transcription, pre-mRNA splicing processes, spliceosome assembly, and promoting undifferentiated endothelial cells.

Alternate Names

75 kDa DNA-pairing protein, ALS6, amyotrophic lateral sclerosis 6, FUS1, fused in sarcoma, fusion (involved in t(12;16) in malignant liposarcoma), fusion gene in myxoid liposarcoma, fusion, derived from t(12;16) malignant liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, Oncogene FUS, Oncogene TLS, POMp75, RNA-binding protein FUS, TLShnRNP-P2, Translocated in liposarcoma protein

Entrez Gene IDs

2521 (Human); 233908 (Mouse)

Gene Symbol

FUS

UniProt

P35637 (Human)

Additional FUS Products

Product Documents for FUS Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FUS Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FUS Antibody - BSA Free

Customer Reviews for FUS Antibody - BSA Free (4)

5 out of 5
4 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • FUS Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human primary fibroblast
    Species: Human
    Verified Customer | Posted 10/31/2020
    FUS in human primary fibroblasts. Primary antibody incubation: 1:1000 in 5% Milk in PBST (0.1% Tween) O/N 4ºC.
    Primary antibody incubation: 1:1000 in 5% Milk in PBST (0.1% Tween) O/N 4ºC. Secondary antibody incubation: 1:5000 1h RT Goat anti-rabbit HRP. ECL+. 1s exposure.
    FUS Antibody - BSA Free NB100-565
  • FUS Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Cortical neurons
    Species: Mouse
    Verified Customer | Posted 02/19/2020
    FUS (NB100-565) (green), b-III Tubulin (white), DAPI (blue). FUS ab dilution: 1:300 in PBST (0.1% triton) + 10% GS O/N 4ºC.
    FUS ab dilution: 1:300 in PBST (0.1% triton) + 10% GS O/N 4ºC.
    FUS Antibody - BSA Free NB100-565
  • FUS Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: cell
    Species: Human
    Verified Customer | Posted 01/30/2019
    DNMT1 WB of human diploid cells
    At least more than 20 μg sample is optimal.
    FUS Antibody - BSA Free NB100-565
  • Name: Simona Darovic
    Application: Immunofluorescence
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 10/15/2013
    FUS Antibody - BSA Free NB100-565

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