G-CSF Antibody (7E4F7) - BSA Free

Novus Biologicals | Catalog # NBP2-52447

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 7E4F7

Format

BSA Free
View all G-CSF Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human G-CSF (AA: 1-207) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse G-CSF Antibody (7E4F7) - BSA Free (NBP2-52447) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for G-CSF Antibody (7E4F7) - BSA Free

Western Blot: G-CSF Antibody (7E4F7)BSA Free [NBP2-52447]

Western Blot: G-CSF Antibody (7E4F7)BSA Free [NBP2-52447]

Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against human CSF3 (AA: 1-207) recombinant protein. (Expected MW is 48.2 kDa)
Immunocytochemistry/ Immunofluorescence: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

Immunocytochemistry/ Immunofluorescence: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

Immunocytochemistry/Immunofluorescence: G-CSF Antibody (7E4F7) [NBP2-52447] - Immunofluorescence analysis of A549 cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

Immunohistochemistry: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of paraffin-embedded esophageal cancer tissues using CSF3 mouse mAb with DAB staining.
Western Blot: G-CSF Antibody (7E4F7)BSA Free [NBP2-52447]

Western Blot: G-CSF Antibody (7E4F7)BSA Free [NBP2-52447]

Western Blot: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis using CSF3 mAb against HEK293 (1) and CSF3 (AA: 1-207)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

Immunohistochemistry: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

Immunohistochemistry: G-CSF Antibody (7E4F7) [NBP2-52447] - Analysis of Hela cells using CSF3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
ELISA: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

ELISA: G-CSF Antibody (7E4F7) - BSA Free [NBP2-52447]

ELISA: G-CSF Antibody (7E4F7) [NBP2-52447] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for G-CSF Antibody (7E4F7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G-CSF

Granulocyte Colony-Stimulating Factor (G-CSF, filgrastim, pegfilgrastim or lenograstim) is a hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage, in a dose-dependent manner. It is produced by a variety of cells including macrophages, monocytes, fibroblasts, endothelial cells and bone marrow stroma in response to specific stimulation by f.ex. endotoxin, TNF-alpha and IFN-gamma. Clinical applications of G-CSF include treatment of leukopenia following cancer therapy and bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is a 18.7 kDa glycoprotein containing 174 amino acid residues.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Gene Symbol

CSF3

Additional G-CSF Products

Product Documents for G-CSF Antibody (7E4F7) - BSA Free

Certificate of Analysis

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Product Specific Notices for G-CSF Antibody (7E4F7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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