G Protein alpha z Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88111

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G Protein alpha z Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for G Protein alpha z Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: G Protein alpha z Antibody [NBP1-88111]

Immunocytochemistry/ Immunofluorescence: G Protein alpha z Antibody [NBP1-88111]

Immunocytochemistry/Immunofluorescence: G Protein alpha z Antibody [NBP1-88111] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Analysis in human cerebral cortex and liver tissues. Corresponding G Protein alpha z RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human pancreas shows strong membranous positivity in islets of langerhans.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111]

Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human cerebellum shows strong cytoplasmic positivity in molecular layer.
G Protein alpha z Antibody - BSA Free Western Blot: G Protein alpha z Antibody - BSA Free [NBP1-88111]

Western Blot: G Protein alpha z Antibody - BSA Free [NBP1-88111]

Analysis in control (vector only transfected HEK293T lysate) and GNAZ over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for G Protein alpha z Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G Protein alpha z

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. G protein z alpha, encoded by the GNAZ gene, is enriched in neural tissue and differs in sequence from other G alpha subunits. Alpha Subunits are encoded in 15 genes and several transcripts are alternatively spliced. Receptors may discriminate between splice variants; whether splice variants are functionally different in regulating effectors is not known. All alpha subunits appear to be palmitoylated near the N-terminus.

Alternate Names

G(x) alpha chain, guanine nucleotide binding protein (G protein), alpha z polypeptide, guanine nucleotide-binding protein G(z) subunit alpha, gz-alpha, transducin alpha

Gene Symbol

GNAZ

Additional G Protein alpha z Products

Product Documents for G Protein alpha z Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for G Protein alpha z Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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