G3BP1 Antibody (2F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00010146-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F3

Format

Azide and BSA Free
View all G3BP1 Primary Antibodies »

Product Specifications

Immunogen

G3BP (AAH06997, 214 a.a. ~ 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRV

Reactivity Notes

Monkey reactivity reported in scientific literature (PMID: 31216693).

Specificity

G3BP - Ras-GTPase-activating protein SH3-domain-binding protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for G3BP1 Antibody (2F3) - Azide and BSA Free

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01]

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01]

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - G3BP monoclonal antibody (M01), clone 2F3 Analysis of G3BP expression in A-431.
Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01]

Immunocytochemistry/ Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01]

Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01]

Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01]

Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 1 ug/ml.
Western Blot: G3BP1 Antibody (2F3) [H00010146-M01]

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01]

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi ( Cat # H00010146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody (M01), clone 2F3 (Cat # H00010146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: G3BP1 Antibody (2F3) [H00010146-M01]

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01]

Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody (M01), clone 2F3.Lane 1: G3BP transfected lysate(52.2 KDa).Lane 2: Non-transfected lysate.
ELISA: G3BP1 Antibody (2F3) [H00010146-M01]

ELISA: G3BP1 Antibody (2F3) [H00010146-M01]

ELISA: G3BP1 Antibody (2F3) [H00010146-M01] - Detection limit for recombinant GST tagged G3BP1 is 0.1 ng/ml as a capture antibody.

Applications for G3BP1 Antibody (2F3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: G3BP1

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Entrez Gene IDs

10146 (Human)

Gene Symbol

G3BP1

OMIM

608431 (Human)

UniProt

AAH06997 (Human)

Additional G3BP1 Products

Product Documents for G3BP1 Antibody (2F3) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for G3BP1 Antibody (2F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for G3BP1 Antibody (2F3) - Azide and BSA Free

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Protocols

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FAQs

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