G3BP1 [p Ser232] Antibody - BSA Free

Novus Biologicals | Catalog # NB100-81931

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all G3BP1 Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human G3BP1 around the phosphorylation site of serine 232 (S-S-SP-P-A).

Modification

p Ser232

Specificity

Detects endogenous levels of G3BP1 only when phosphorylated at serine 232.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G3BP1 [p Ser232] Antibody - BSA Free

Western Blot: G3BP1 [p Ser232] Antibody [NB100-81931]

Western Blot: G3BP1 [p Ser232] Antibody [NB100-81931]

Western Blot: G3BP1 [p Ser232] Antibody [NB100-81931] - Analysis of extracta from 293 cells untreated or treated with starvation using G3B P-1 Antibody.
G3BP1 [p Ser232] Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: G3BP1 [p Ser232] Antibody - BSA Free [NB100-81931] -

Immunocytochemistry/ Immunofluorescence: G3BP1 [p Ser232] Antibody - BSA Free [NB100-81931] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-81931.
G3BP1 [p Ser232] Antibody - BSA Free

Immunohistochemistry-Paraffin: G3BP1 [p Ser232] Antibody [NB100-81931] -

Immunohistochemistry-Paraffin: G3BP1 [p Ser232] Antibody [NB100-81931] - Analysis of Human Breast Carcinoma tissue using NB100-81931 (left) or the same antibody preincubated with blocking peptide (right)

Applications for G3BP1 [p Ser232] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G3BP1

G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternate Names

ATP-dependent DNA helicase VIII, EC 3.6.1, EC 3.6.4.12, EC 3.6.4.13, G3BP-1, G3BPRas-GTPase-activating protein SH3-domain-binding protein, GAP binding protein, GAP SH3 domain-binding protein 1, GTPase activating protein (SH3 domain) binding protein 1, hDH VIII, HDH-VIII, MGC111040, ras GTPase-activating protein-binding protein 1, RasGAP-associated endoribonuclease G3BP

Entrez Gene IDs

10146 (Human)

Gene Symbol

G3BP1

UniProt

Q13283 (Human)

Additional G3BP1 Products

Product Documents for G3BP1 [p Ser232] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for G3BP1 [p Ser232] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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