GABA-AR alpha 1 Antibody - Azide Free

Novus Biologicals | Catalog # NB300-191

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Human (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all GABA-A R alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P62813

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22927941)

Localization

Membrane; multi pass membrane protein.

Specificity

Specific for endogenous levels of the ~51 kDa alpha1-subunit of the GABAA receptor. Immunolabeling is absent in alpha1-subunit knockout animals.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-AR alpha 1 Antibody - Azide Free

Western Blot: GABA-AR alpha 1 Antibody [NB300-191]

Western Blot: GABA-AR alpha 1 Antibody [NB300-191]

Western Blot: GABA-A R alpha 1 Antibody [NB300-191] - Mouse forebrain lysates from Wild Type (WT) and alpha 1-knockout (K/O) animals showing specific immunolabeling of the -51 kDa alpha 1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 1-knockout animals.

Applications for GABA-AR alpha 1 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:100

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 22927941). Use in immunoprecipitation reported in scientific literature (PMID: 17404840).

Reviewed Applications

Read 1 review rated 5 using NB300-191 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R alpha 1

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide

Alternate Names

EJM, GABAAR alpha 1, GABAARa1, GABRA1

Entrez Gene IDs

2554 (Human); 14394 (Mouse); 29705 (Rat); 780973 (Bovine)

Gene Symbol

GABRA1

UniProt

P62813 (Rat)

Additional GABA-A R alpha 1 Products

Product Documents for GABA-AR alpha 1 Antibody - Azide Free

Certificate of Analysis

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Download SDS

Product Specific Notices for GABA-AR alpha 1 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
GABA Receptors

Citations for GABA-AR alpha 1 Antibody - Azide Free

Customer Reviews for GABA-AR alpha 1 Antibody - Azide Free (1)

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  • GABA-A R alpha 1 Antibody
    Name: Daniel Popoola
    Application: Western Blot
    Sample Tested: brain and spinal cord and brain
    Species: Rat
    Verified Customer | Posted 03/21/2017
    Whole-cell homogenates of brain tissue from adolescent and adult rats.
    GABA-AR alpha 1 Antibody - Azide Free NB300-191

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Protocols

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FAQs for GABA-AR alpha 1 Antibody - Azide Free

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  • Q: Are there neutralizing peptides for this antibody?

    A: There is no neutralizing peptide available for this antibody.

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