GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43812
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL2911
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q99259, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free
![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Western Blot: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-28220251273710.jpg "Western Blot: GAD1/GAD67 Antibody [NBP3-43812]")
Western Blot: GAD1/GAD67 Antibody [NBP3-43812]
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-282202512195413.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of human cerebellum shows strong immunoreactivity in Purkinje cells, as well as in fibers in granular and molecular layers.![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-282202512303860.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of human cerebral cortex shows immunoreactivity in neuropil, as well as some neuronal cells bodies.![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-282202513112812.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of human pancreas shows absence of immunoreactivity (negative control).![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-282202513415691.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of rat cerebral cortex shows strong positivity in GABAergic synapses on pyramidal neurons.![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-28220251324512.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of mouse cerebellum shows positivity in GABAergic neural fibers.![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-282202513415673.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of rat globus pallidus shows strong immunoreactivity in GABAergic neural fibers.![GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]](https://resources.rndsystems.com/images/products/nbp3-43812_mouse-gad1-gad67-mab-cl2911-azide-and-bsa-free-282202513314688.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody [NBP3-43812]
Staining of mouse substantia nigra shows strong immunoreactivity in GABAergic neural processes.Applications for GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: GAD1/GAD67
Long Name
Glutamate Decarboxylase 1 (Brain, 67kDa)
Alternate Names
GAD67
Gene Symbol
GAD1
Additional GAD1/GAD67 Products
Product Documents for GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free and earn rewards!
Have you used GAD1/GAD67 Antibody (CL2911) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
Synaptic Neurotransmission Pathways: GABAergic Inhibition
