GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13716
Recombinant Monoclonal Antibody
![Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-HL1096-Western-Blot-NBP3-13716-img0001.jpg "Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]")
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Feline, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1096 expressed in HEK293
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAD1/GAD67. The exact sequence is proprietary.
Reactivity Notes
Cat (100%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free
Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]
Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-HL1096-Immunohistochemistry-Paraffin-NBP3-13716-img0004.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-HL1096-Immunohistochemistry-Paraffin-NBP3-13716-img0002.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a nerual marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min![Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]](https://resources.rndsystems.com/images/products/GAD1-GAD67-Antibody-HL1096-Immunohistochemistry-Paraffin-NBP3-13716-img0003.jpg "Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]")
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716]
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] - GAD1/GAD67 antibody [HL1096] detects GAD1/GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GAD1/GAD67 stained by GAD1/GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
GAD67 antibody [HL1096] detects GAD67 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: GAD67 stained by GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:200.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C
Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog cerebellum.
GAD67 stained by GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: GAD1/GAD67 Antibody (HL1096) [NBP3-13716] -
GAD67 antibody [HL1096] detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat cerebellum.
GAD67 stained by GAD67 antibody [HL1096] (NBP3-13716) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Assay dependent
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GAD1/GAD67
Long Name
Glutamate Decarboxylase 1 (Brain, 67kDa)
Alternate Names
GAD67
Gene Symbol
GAD1
Additional GAD1/GAD67 Products
Product Documents for GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free and earn rewards!
Have you used GAD1/GAD67 Antibody (HL1096) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...
Associated Pathways
Synaptic Neurotransmission Pathways: GABAergic Inhibition
