![Western Blot: GADD34 Antibody [NB100-778]](https://resources.rndsystems.com/images/products/GADD34-Antibody-Western-Blot-NB100-778-img0004.jpg "Western Blot: GADD34 Antibody [NB100-778]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-AAALDLSGRRG corresponding to C-Terminus according to NP_055145.3.
Marker
ER Stress Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for GADD34 Antibody
Western Blot: GADD34 Antibody [NB100-778]
Western Blot: GADD34 Antibody [NB100-778] - Staining of HepG2 (A) and negative control KLY (B) lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]](https://resources.rndsystems.com/images/products/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0007.jpg "Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]")
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]
Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).![Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778]](https://resources.rndsystems.com/images/products/GADD34-Antibody-Immunohistochemistry-Paraffin-NB100-778-img0003.jpg "Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778]")
Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778]
Immunohistochemistry-Paraffin: GADD34 Antibody [NB100-778] - (2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.![Flow Cytometry: GADD34 Antibody [NB100-778]](https://resources.rndsystems.com/images/products/GADD34-Antibody-Flow-Cytometry-NB100-778-img0008.jpg "Flow Cytometry: GADD34 Antibody [NB100-778]")
Flow Cytometry: GADD34 Antibody [NB100-778]
Flow Cytometry: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]](https://resources.rndsystems.com/images/products/GADD34-Antibody-Immunocytochemistry-Immunofluorescence-NB100-778-img0006.jpg "Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]")
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody [NB100-778]
Immunocytochemistry/Immunofluorescence: GADD34 Antibody [NB100-778] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).Applications for GADD34 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
2 - 4 ug/ml
Immunohistochemistry-Paraffin
2-4ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.3-1 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in HepG2 lysates (calculated MW of 73.4 kDa band according to NP_055145.2). IHC-P: Human liver shows cytoplasmic staining consistent with ER in the hepatocytes.
Reviewed Applications
Read 1 review rated 4 using NB100-778 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GADD34
Long Name
Protein phosphatase 1 regulatory subunit 15A
Alternate Names
PPP1R15A
Entrez Gene IDs
23645 (Human)
Gene Symbol
PPP1R15A
UniProt
Additional GADD34 Products
Product Documents for GADD34 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GADD34 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for GADD34 Antibody
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1 Customer Rating
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Application: Western BlotSample Tested: whole cell lysate, Sample Amount: 40 ugSpecies: OtherVerified Customer | Posted 12/09/2009
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for GADD34 Antibody
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Q: We are interested in your items, NB100-778. Would you please let us know the % of predicted cross reactivity to drosophila based on sequence homology? http://www.uniprot.org/uniprot/Q9W1E4
A: Unfortunately, the immunogen of NB100-778 is only about 36% homologous to the corresponding region of the Drosophila sequence. Based on this information, I would not recommend this product for use in Drosophila.
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