Galectin-3 Antibody (GT12712) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13641

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT12712

Format

Azide and BSA Free
View all Galectin-3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Galectin-3. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Galectin-3 Antibody (GT12712) - Azide and BSA Free

Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]

Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]

Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Wild-type (WT) and Galectin-3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641]

Immunocytochemistry/ Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641]

Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (GT12712) [NBP3-13641] - Galectin-3 antibody [GT12712] detects Galectin-3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Galectin-3 stained by Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]

Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641]

Western Blot: Galectin-3 Antibody (GT12712) [NBP3-13641] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Sandwich ELISA: Galectin-3 Antibody (GT12712) [NBP3-13641] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using NBP3-13641 as capture antibody at concentration of 5 ug/mL and NBP2-16590 as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) (NBP2-19301) was diluted at 1:10000 and used to detect the primary antibody.
Galectin-3 Antibody (GT12712)

Immunohistochemistry-Paraffin: Galectin-3 Antibody (GT12712) [NBP3-13641] -

Immunohistochemistry-Paraffin: Galectin-3 Antibody (GT12712) [NBP3-13641] - Galectin-3 antibody [GT12712] detects Galectin-3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Galectin-3 stained by Galectin-3 antibody [GT12712] (NBP3-13641) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Galectin-3 Antibody (GT12712) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Galectin-3 Antibody (GT12712) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Galectin-3 Antibody (GT12712) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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