GALT Antibody (4C11) - Azide and BSA Free

Novus Biologicals | Catalog # H00002592-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C11

Format

Azide and BSA Free
View all GALT Primary Antibodies »

Product Specifications

Immunogen

GALT (AAH15045, 1 a.a. ~ 379 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRSGTDPQQRQQASEADAAAATFRANDHQHIRYNPLQDEWVLVSAHRMKRPWQGQVEPQLLKTVPRHDPLNPLCPGAIRANGEVNPQYDSTFLFDNDFPALQPDAPSPGPSDHPLFQAKSARGVCKVMCFHPWSDVTLPLMSVPEIRAVVDAWASVTEELGAQYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPDIAQREERSQQAYKSQHGEPLLMEYSRQELLRKERLVLTSEHWLVLVPFWATWPYQTLLLPRRHVRRLPELTPAERDDLASIMKKLLTKYDNLFETSFPYSMGWHGAPTGSEAGANWDHWQLHAHYYPPLLRSATVRKFMVGYEMLAQAQRDLTPEQAAERLRALPEVHYHLGQKDRETATIA

Reactivity Notes

Rat reactivity reported from customer review.

Specificity

GALT - galactose-1-phosphate uridylyltransferase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GALT Antibody (4C11) - Azide and BSA Free

Western Blot: GALT Antibody (4C11) [H00002592-M01]

Western Blot: GALT Antibody (4C11) [H00002592-M01]

Western Blot: GALT Antibody (4C11) [H00002592-M01] - Analysis of GALT expression in transfected 293T cell line by GALT monoclonal antibody (M01), clone 4C11.Lane 1: GALT transfected lysate(43 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: GALT Antibody (4C11) [H00002592-M01]

Immunohistochemistry-Paraffin: GALT Antibody (4C11) [H00002592-M01]

Immunohistochemistry-Paraffin: GALT Antibody (4C11) [H00002592-M01] - Analysis of monoclonal antibody to GALT on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
Western Blot: GALT Antibody (4C11) [H00002592-M01]

Western Blot: GALT Antibody (4C11) [H00002592-M01]

Western Blot: GALT Antibody (4C11) [H00002592-M01] - GALT monoclonal antibody (M01), clone 4C11 Analysis of GALT expression in K-562.
Sandwich ELISA: GALT Antibody (4C11) [H00002592-M01] - Detection limit for recombinant GST tagged GALT is approximately 0.3ng/ml as a capture antibody.

Applications for GALT Antibody (4C11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:34964137) Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00002592-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GALT

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Long Name

galactose-1-phosphate uridylyltransferase

Alternate Names

EC 2.7.7.12, Gal-1-P uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Entrez Gene IDs

2592 (Human)

Gene Symbol

GALT

OMIM

230 (Human)

UniProt

AAH15045 (Human)

Additional GALT Products

Product Documents for GALT Antibody (4C11) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GALT Antibody (4C11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GALT Antibody (4C11) - Azide and BSA Free

Customer Reviews for GALT Antibody (4C11) - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Column fractions, Sample Amount: 100 ng
    Species: Other
    Verified Customer | Posted 12/21/2009

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Protocols

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FAQs

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