gamma Adducin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-34017

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all gamma Adducin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ASSVSQIQSQTQSPQNVPEKLEENHELFSKSFISMEVPVMVVNGKDDMHDVEDELAKRVSRLSTSTTIENIEITIKSPEKIEEVLSPE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for gamma Adducin Antibody - BSA Free

Western Blot: gamma Adducin Antibody [NBP2-34017]

Western Blot: gamma Adducin Antibody [NBP2-34017]

Western Blot: gamma Adducin Antibody [NBP2-34017] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-229
Immunocytochemistry/ Immunofluorescence: gamma Adducin Antibody [NBP2-34017]

Immunocytochemistry/ Immunofluorescence: gamma Adducin Antibody [NBP2-34017]

Immunocytochemistry/Immunofluorescence: gamma Adducin Antibody [NBP2-34017] - Staining of human cell line HEK 293 shows localization to plasma membrane.
Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
Immunohistochemistry: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry: gamma Adducin Antibody [NBP2-34017] - Staining of human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP2-34017 antibody. Corresponding ADD3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017] - Staining of human cerebral cortex shows strong positivity in neuropil and glial cells.
Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017] - Staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017]

Immunohistochemistry-Paraffin: gamma Adducin Antibody [NBP2-34017] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for gamma Adducin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Adducin

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta and gamma). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. The cortical actin cytoskeletal network is lost during apoptosis and adducins are central in the cortical actin network organization. Adducin alpha is a cytoskeletal protein involved with sodium-pump activity in the renal tubule and is associated with hypertension. The expression of Adducin alpha and Adducin gamma is ubiquitous in contrast to the restricted expression of Adducin beta. Adducin beta is expressed at high levels in brain and hematopoietic tissues, such as bone marrow in humans, and spleen in mice.

Alternate Names

ADDLAdducin-like protein 70, adducin 3 (gamma), gamma-adducin

Gene Symbol

ADD3

UniProt

Q9UEY8 (Human)

Additional gamma Adducin Products

Product Documents for gamma Adducin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for gamma Adducin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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