GAP-43 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-41123

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline, Hamster, Primate

Cited:

Human, Mouse, Rat, Hamster

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

BSA Free
View all GAP-43 Primary Antibodies »

Product Specifications

Immunogen

GAP-43 from rat brain [Uniprot: P07936]

Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAP-43 Antibody - BSA Free

Immunohistochemistry: GAP-43 Antibody [NBP1-41123]

Immunohistochemistry: GAP-43 Antibody [NBP1-41123]

Immunohistochemistry: GAP-43 Antibody [NBP1-41123] - Coronal sections of the developing (A-H) eye at (A-D) E14.5, (E-H) E17.5. were immunostained for (A-H) EVA1C and (A-H) GAP43. All sections were counterstained with DAPI. EVA1C was strongly co-expressed with GAP43 by retinal cell ganglionic axons (A-H) as they exit the retina and form the optic nerve (arrowheads). Scale bars represent 100 uM. PLoS One. 2013 Sep 9;8(9):e74115. doi: 10.1371/journal.pone.0074115.
Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP1-41123]

Immunocytochemistry/ Immunofluorescence: GAP-43 Antibody [NBP1-41123]

Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP1-41123] - Staining of a mature retinal ganglion cell demonstrating axon outgrowth.
Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123]

GAP-43-Antibody-Western-Blot-NBP1-41123-img0008.jpg
Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123] - Analysis of GAP43 in human brain protein
Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123]

Western Blot: GAP-43 Antibody [NBP1-41123] - Total protein from Human, Mouse and Rat brain, and SHSY-5Y cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-GAP43 in 1% non-fat milk in TBST and detected with an anti-sheep HRP secondary antibody using chemiluminescence.

Applications for GAP-43 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 28112682)

Immunocytochemistry/ Immunofluorescence

1:50-1:1000

Immunohistochemistry

1:400-1:1000

Immunohistochemistry-Frozen

1:400-1:1000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24686445)

Western Blot

1:1000
Application Notes
In Western blot, a band can be seen at 43-48 kDa.

Reviewed Applications

Read 1 review rated 4 using NBP1-41123 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAP-43

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the synaptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axonal as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease.

Long Name

Growth Associated Protein 43

Alternate Names

B-50, Basp2, GAP43, Neuromodulin

Entrez Gene IDs

14432 (Mouse)

Gene Symbol

GAP43

Additional GAP-43 Products

Product Documents for GAP-43 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GAP-43 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GAP-43 Antibody - BSA Free

Customer Reviews for GAP-43 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Rat olfactory epithelium tissue lysate
    Species: Rat
    Verified Customer | Posted 05/28/2015
    Rat Olfactory epthelium tissue, 10ug/lane (lane1: saline, lane2: ZnSO4)
    GAP-43 Antibody - BSA Free NBP1-41123

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Protocols

View specific protocols for GAP-43 Antibody - BSA Free (NBP1-41123):


Western Blot Protocol
1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 25 ug of total protein per lane.
2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
3. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
4. Rinse the blot in TBS for approximately 5 minutes.
5. Block the membrane using 5% NFDM + 1% BSA in TBS + Tween, 1 hour at RT.
6. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
7. Dilute the anti-GAP43 primary antibody (NBP1-41123) in blocking buffer and incubate 1 hour at room temperature.
8. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each.
9. Apply the diluted sheep-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer [TBS + 0.1% Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%, provided it does not interfere with antibody-antigen binding.

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FAQs for GAP-43 Antibody - BSA Free

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  • Q: Can you confirm the tested application of NBP1-41123 includes Immunohistochemistry on Formalin-Fixed Paraffin Embedded sections?

    A: NBP1-41123 has only been validated in IHC on frozen sections at this time. However, NB300-143 has been validated in IHC on both frozen and paraffin-embedded tissues.

  • Q: Could you tell us the sequence homology between the immunogen of this antibody and the GAP43 protein of canine?

    A: The immunogen for NB300-143 shares 82% similarity with canine. NBP1-41123 may be a better choice. It is known to work in dog and in IHC. It has also been published with 10 times in IHC and some of these publications almost certainly used paraffin-embedded tissues.

  • Q: Can you confirm the tested application of NBP1-41123 includes Immunohistochemistry on Formalin-Fixed Paraffin Embedded sections?

    A: NBP1-41123 has only been validated in IHC on frozen sections at this time. However, NB300-143 has been validated in IHC on both frozen and paraffin-embedded tissues.

  • Q: Could you tell us the sequence homology between the immunogen of this antibody and the GAP43 protein of canine?

    A: The immunogen for NB300-143 shares 82% similarity with canine. NBP1-41123 may be a better choice. It is known to work in dog and in IHC. It has also been published with 10 times in IHC and some of these publications almost certainly used paraffin-embedded tissues.

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