GAPDH Antibody (GA1R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-37828

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bacteria, Chicken, Hamster, Insect, Rabbit, Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GA1R

Format

Azide and BSA Free
View all GAPDH Primary Antibodies »

Product Specifications

Immunogen

This GAPDH protein was developed against recombinant GAPDH

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 17868880). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This GAPDH antibody detects GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, hamster, and chicken samples. This product has been successfully used in Western blot, ELISA, and immunofluorescence.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GAPDH Antibody (GA1R) - Azide and BSA Free

Western Blot: GAPDH Antibody (GA1R)Azide and BSA Free [NBP2-37828]

Western Blot: GAPDH Antibody (GA1R)Azide and BSA Free [NBP2-37828]

Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3). Theoretical molecular weight: 36 kDa.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) in HeLa. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were probed with a GAPDH monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.
Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of paraffin-embedded human breast carcinoma (right) compared with a negative control without primary antibody (left).
Flow Cytometry: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Flow Cytometry: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in Hela cells (green) compared to an isotype control (blue).
Western Blot: GAPDH Antibody (GA1R)Azide and BSA Free [NBP2-37828]

Western Blot: GAPDH Antibody (GA1R)Azide and BSA Free [NBP2-37828]

Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). Theoretical molecular weight: 36 kDa.
Western Blot: GAPDH Antibody (GA1R)Azide and BSA Free [NBP2-37828]

Western Blot: GAPDH Antibody (GA1R)Azide and BSA Free [NBP2-37828]

Western Blot: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. GAPDH theoretical molecular weight: 36 kDa.
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of mouse colon tissue (right) compared with a negative control without primary antibody (left).
Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Immunohistochemistry-Paraffin: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis showing staining in the nucleus and cytoplasm of human kidney tissue (right) compared with a negative control without primary antibody (left).
Flow Cytometry: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Flow Cytometry: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in NIH-3T3 cells (green) compared to an isotype control (blue).
Flow Cytometry: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Flow Cytometry: GAPDH Antibody (GA1R) - Azide and BSA Free [NBP2-37828]

Flow Cytometry: GAPDH Antibody (GA1R) [NBP2-37828] - Analysis of GAPDH in MCF-7 cells (green) compared to an isotype control (blue).

Applications for GAPDH Antibody (GA1R) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

2 ug

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Immunohistochemistry

1:50 - 1:500

Immunohistochemistry-Paraffin

1:50 - 1:500

Western Blot

1:1000 - 1:10000
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GAPDH

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase

Alternate Names

G3PDH

Gene Symbol

GAPDH

Additional GAPDH Products

Product Documents for GAPDH Antibody (GA1R) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for GAPDH Antibody (GA1R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for GAPDH Antibody (GA1R) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GAPDH Antibody (GA1R) - Azide and BSA Free

Showing  1 - 5 of 8 FAQs Showing All

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

Showing  1 - 5 of 8 FAQs Showing All
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